We wish to synthesize two type of novel polymers based on proven hypotheses and promising preliminary data. First, we propose the synthesis of multiblock copolymers which have hydrophilic-hydrophobic microdomain surfaces. Secondly, we will synthesis triblock-copolymers containing a hydrophobic block A (commercially available medical polymer), a hydrophilic block B(minimizing protein adsorption and platelet interactions) and a block C consisting of heparin (preventing fibrin formation). It has been shown by preliminary experiments that a multiblock copolymer, polyethylene oxide-polystyrene (PEO-PS) showed suppression of platelet aggregation. Triblock copolymers, PS-PEO-Heparin was biologically active when it was coated on PS surfaces and tested in vitro. The synthesized multiblock copolymers and triblock copolymers will be characterized using TEM, FTIR-ATR and contact angle measurement. Their nonthrombogenicity will be evaluated in vitro assay methods and in vivo using AV shunt rabbits and vascular implants in dogs.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL017623-16
Application #
3335390
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Project Start
1974-09-01
Project End
1991-03-31
Budget Start
1989-09-01
Budget End
1991-03-31
Support Year
16
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Utah
Department
Type
Schools of Pharmacy
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Kim, S W; Jacobs, H (1996) Design of nonthrombogenic polymer surfaces for blood-contacting medical devices. Blood Purif 14:357-72
Gutowska, A; Bae, Y H; Jacobs, H et al. (1995) Heparin release from thermosensitive polymer coatings: in vivo studies. J Biomed Mater Res 29:811-21
Castner, D G; Ratner, B D; Grainger, D W et al. (1992) Surface characterization of 2-hydroxyethyl methacrylate/styrene copolymers by angle-dependent X-ray photoelectron spectroscopy and static secondary ion mass spectrometry. J Biomater Sci Polym Ed 3:463-80
Grainger, D W; Knutson, K; Kim, S W et al. (1990) Poly(dimethylsiloxane)-poly(ethylene oxide)-heparin block copolymers. II: Surface characterization and in vitro assessments. J Biomed Mater Res 24:403-31
Piao, A Z; Nojiri, C; Park, K D et al. (1990) Synthesis and characterization of poly(dimethylsiloxane)-poly(ethylene oxide)-heparin CBABC type block copolymers. J Biomater Sci Polym Ed 1:299-313
Grainger, D W; Okano, T; Kim, S W et al. (1990) Poly(dimethylsiloxane)-poly(ethylene oxide)-heparin block copolymers. III: Surface and bulk compositional differences. J Biomed Mater Res 24:547-71
Grainger, D W; Nojiri, C; Okano, T et al. (1989) In vitro and ex vivo platelet interactions with hydrophilic-hydrophobic poly(ethylene oxide)-polystyrene multiblock copolymers. J Biomed Mater Res 23:979-1005
Grainger, D W; Kim, S W; Feijen, J (1988) Poly(dimethylsiloxane)-poly(ethylene oxide)-heparin block copolymers. I. Synthesis and characterization. J Biomed Mater Res 22:231-49
Kim, S W (1987) Platelet adhesion and prevention at blood-polymer interface. Artif Organs 11:228-36
Nojiri, C; Okano, T; Grainger, D et al. (1987) Evaluation of nonthrombogenic polymers in a new rabbit A-A shunt model. ASAIO Trans 33:596-601