Abstract

The regulation of intracellular Calcium is essential for Cardiac Cell Function. One of the important subcellular organelles responsible for a large part of calcium intracellular regulation is the sarcoplasmic reticulum. Calcium uptake into cardiac sarcoplasmic reticulum is modulated by the beta adrenergic amines cascade reaction which is mediated by the 22K dalton protein phospholamban and possibly a 6K dalton protein. Calmodulin is also involved in the regulation of phospholamban phosphorylation and thus affecting Ca2+ uptake. This proposal is directed towards the elucidation of molecular mechanisms involved in the modulation of Ca2+ uptake due to the phosphorylation of phospholamban by c-AMP dependent protein kinase and Ca2+ calmodulin systems. Our laboratory has purified phospholamban and ATPase and thus can carry out reconstitution experiments to understand the role of phospholamban and 6K dalton protein in Ca2+ uptake.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL030677-02
Application #
3341712
Study Section
(SSS)
Project Start
1984-09-01
Project End
1987-08-31
Budget Start
1985-09-01
Budget End
1986-08-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Maryland Baltimore
Department
Type
Schools of Medicine
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201

  Publications

Shamoo, A E; Joshi, N B; Lockwich, T (1988) Regulation of cardiac sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPase. Mol Cell Biochem 82:45-9
Shamoo, A E; Joshi, N B (1988) Regulation of the peptide region of the calcium and ATP sites of the calcium pump protein. Prog Clin Biol Res 252:141-8
Ambudkar, I S; Abdallah, E S; Shamoo, A E (1988) Lysophospholipid-mediated alterations in the calcium transport systems of skeletal and cardiac muscle sarcoplasmic reticulum. Mol Cell Biochem 79:81-9
Gangola, P; Shamoo, A E (1987) Characterization of (Ca2+ + Mg2+)-ATPase of sarcoplasmic reticulum by laser-excited europium luminescence. Eur J Biochem 162:357-63
Joshi, N B; Shamoo, A E (1987) Binding of Eu3+ to cardiac sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPase-laser excited Eu3+ spectroscopic studies. Biophys J 51:185-91
Gangola, P; Shamoo, A E (1986) Synthesis and characterization of a peptide segment of (Ca2+ + Mg2+)-ATPase. A candidate for calcium transport site. J Biol Chem 261:8601-3
Ambudkar, I S; Fanfarillo, D T; Shamoo, A E (1986) Phospholamban involvement in the maintenance of basal calcium transport in cardiac sarcoplasmic reticulum. Membr Biochem 6:327-46
Herrmann, T R; Gangola, P; Shamoo, A E (1986) Estimation of inter-binding-site distances in sarcoplasmic reticulum (Ca2+ + Mg2+)-ATPase using Eu(III) luminescence energy transfer. Eur J Biochem 158:555-60
Herrmann, T R; Jayaweera, A R; Shamoo, A E (1986) Interaction of europium(III) with phospholipid vesicles as monitored by laser-excited europium(III) luminescence. Biochemistry 25:5834-8
Sassaroli, M; Fronticelli, C; Kowalczick, J et al. (1986) Librational motions of membrane-embedded Ca2+-ATPase of sarcoplasmic reticulum labeled with fluorescein isothiocyanate. Biophys Chem 25:283-6