Sepsis continues to be a major cause of death following shock, trauma and burns. Cardiac performance during sepsis has been intensively investigated with disparate results. Recently, the use of load independent assessments of cardiac contractility such as end-systolic pressure-volume relationship have indicated depressed inotropity during acute endotoxemia. Our previous studies indicate that animals which survive acute endotoxemia exhibited no depression of cardiac contractility whereas nonsurviving animals exhibited a marked depression. These data suggest that cardiac contractile failure could be a determinant of nonsurvival following acute endotoxemia. Furthermore, this study revealed that depressed cardiac contractile function was associated with a relative reduction in myocardial blood flow. Since clinical sepsis can persist for several days, this study will use a method to produce chronic endotoxemia. In our pilot studies, we implanted an endotoxin loaded osmotic infusion pump into a chronically instrumented pig so that endotoxin was infused i.v. over 4 days. This pig exhibited a 2 day """"""""hyperdynamic"""""""" state followed by a """"""""hypodynamic"""""""" state. The """"""""hyperdynamic"""""""" state was characterized by elevated systolic pressure, dP/dt and rate and reduced contractility as indicated by end systolic pressure-wall thickness relationship. This project will study the effect of chronic endotoxemia upon cardiac systolic and diastolic function in chronically instrumented pigs. We will characterize the relationship of global and regional myocardial perfusion to systolic and diastolic function. We will then test the hypothesis that the compromise of cardiac systolic and diastolic function is caused by myocardial underperfusion by determining if pharmacological augmentation of myocardial perfusion via administration of alpha adrenergic receptor blockade or direct coronary vasodilation (via dipyridamole) will attentuate the deterioration of cardiac function induced by chronic endotoxemia. Future studies will determine if reversal of myocardial flow deficit and presumably restoration of cardiac function can forestall the conversion of """"""""hyperdynamic"""""""" to """"""""hypodynamic"""""""" endotoxemia.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
1R01HL035825-01
Application #
3350184
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Project Start
1985-12-01
Project End
1988-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Albany Medical College
Department
Type
Schools of Medicine
DUNS #
City
Albany
State
NY
Country
United States
Zip Code
12208
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Singh, A; Lee, K J; Lee, C Y et al. (1989) Relation between myocardial glutathione content and extent of ischemia-reperfusion injury. Circulation 80:1795-804
Goldfarb, R D; Lee, K J; Dziuban Jr, S W (1989) Variation in end-systolic pressure-diameter relationship using dP/dtmin or P/Dmax as a definition of end-systole in chronic endotoxemic pigs. Circ Shock 28:109-19
Lee, K J; Dziuban Jr, S W; van der Zee, H et al. (1988) Cardiac function and coronary flow in chronic endotoxemic pigs. Proc Soc Exp Biol Med 189:245-52
Lee, K; van der Zee, H; Dziuban Jr, S W et al. (1988) Left ventricular function during chronic endotoxemia in swine. Am J Physiol 254:H324-30