This proposal examines the role of neutrophils, lung-resident macrophages, and nitric oxide in pulmonary ischemia reperfusion (I/R) injury. Three specific hypotheses will be tested. (1) pulmonary macrophages play a major role in lung I/R injury, and this injury is increased by a relative lack of NO which normally suppresses macrophage cytokine production; (2) Activation of adenosine 2A (A2A) receptors can substantially reduce lung I/R injury; the role of NO in mediating the biological effects of A2A receptor activation in lung I/R injury will be studied; (3) reperfusion injury increases the risk of subsequent acute and chronic rejection. Studies to test these hypotheses will be performed in a blood-perfused rabbit lung model of I/R, a rat model of lung I/R injury, a mouse isolated lung I/R model, and a porcine lung transplantation model.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL056093-08
Application #
7046895
Study Section
Surgery, Anesthesiology and Trauma Study Section (SAT)
Program Officer
Reynolds, Herbert Y
Project Start
1997-07-01
Project End
2007-04-30
Budget Start
2006-05-01
Budget End
2007-04-30
Support Year
8
Fiscal Year
2006
Total Cost
$252,913
Indirect Cost
Name
University of Virginia
Department
Surgery
Type
Schools of Medicine
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
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