The overall goal of this project is to determine whether autotransplantation of skeletal myoblasts into scarred or failing myocardium will lead to long term survival of the myoblast and improvement in systolic and diastolic contractile function. The Principal Investigator plans to use a model of cryoablation injury to the ventricle injecting the myoblasts into the area of damage. In the first aim she will develop a method of labeling the transplanted cells so that they could be detected late and to facilitate determination of longevity. In the second aim, she will determine regional wall motion abnormalities in the area of myoblast transplantation and determine the microenvironment to which the cells are transplanted. In the third aim, she will determine whether the viability of harvested skeletal myoblasts is altered by the presence of congestive heart failure. She will also test the efficacy of myoblast transplantation in a model of coronary ischemia.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Project (R01)
Project #
5R01HL063346-03
Application #
6390498
Study Section
Surgery and Bioengineering Study Section (SB)
Program Officer
Reinlib, Leslie
Project Start
1999-08-01
Project End
2003-07-31
Budget Start
2001-08-01
Budget End
2002-07-31
Support Year
3
Fiscal Year
2001
Total Cost
$318,096
Indirect Cost
Name
Duke University
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
071723621
City
Durham
State
NC
Country
United States
Zip Code
27705
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