The proposed research will use recently developed maps of regional activity patterns associated with behaviors that do and do not entail explicit learning, to test the hypothesis that proteolytic and synthetic chemistries linked to long term potentiation (LTP) are activated in situ during learning. Behavioral groups include rats at different stages in olfactory discrimination learning, recently engaged in copulation, or having received an IP saline injection; analysis will focus on subfields of hippocampus and amygdala.
The first aim i s to map cfos mRNA expression to identify regions activated during learning: the analysis will complete the evaluation of cfos expression in 8 behavioral groups.
The second aim i s to test the hypothesis that mRNA expression for the cytoskeleton-associated protein Arc increases in regions activated during behavior and that this effect will be greater in conditions associated with explicit learning as compared to conditions that are not. In addition, effects on Arc protein content will be evaluated.
The third aim i s to test the hypothesis that neurotrophin mRNA expression covaries with neuronal activity in a subset of areas that exhibit changes in c-fos expression, and most particularly in areas activated with learning as opposed to familiar behaviors.
The fourth aim i s to use antisera specific for spectrin fragments deriving from calpain activity to test the hypothesis that the protease is stimulated in dendritic spines in association with LTP and in select hippocampal and amygdala areas during learning. The chemistries to be evaluated (calpain activity, neurotrophin and Arc expression) are argued to be critical for certain forms of synaptic plasticity, and are candidates for involvement in memory encoding. The proposed work will provide a first test for their presence in regions engaged learning behaviors.

Agency
National Institute of Health (NIH)
Institute
National Institute of Mental Health (NIMH)
Type
Research Project (R01)
Project #
5R01MH057947-03
Application #
6185852
Study Section
Neuropharmacology and Neurochemistry Review Committee (NPNC)
Program Officer
Asanuma, Chiiko
Project Start
1998-07-01
Project End
2002-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
3
Fiscal Year
2000
Total Cost
$175,413
Indirect Cost
Name
University of California Irvine
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697