Both postprandial oxidative stress and derangements in carbohydrate (CHO) and lipid metabolism have been implicated in the development of complications in type 2 DM. Yet the ability of aerobic exercise to improve these derangements has not been fully explored. This study will examine the effects of an acute bout of aerobic exercise (30 minutes of treadmill walking at 65% VO2Max) in a group of physically untrained subjects with younger-onset type 2 DM and non-DM controls on: (1) CHO and lipid metabolism (glucose, insulin, C-peptides and triglycerides) following 3 meals; (2) postprandial oxidative stress (total MDA, F2 Isoprostanes, total hydroperoxides, vitamin A, E and carotenoids) and LDL susceptibility to oxidation (lag time, maximal conjugated diene formation) following 3 meals; and (3) oxidative stress and LDL susceptibility to oxidation 30 minutes after an acute bout of aerobic exercise. In addition, the relationship between baseline insulin sensitivity, antioxidant status, VO2Max and exercise-induced changes in oxidative stress, CHO and lipid metabolism will be explored. The study will include 35 subjects with type 2 DM (ages 18-40) with duration of diabetes (<7 years) and 35 non-DM controls who will participate in a nonexercise (NE) and an exercise (E) protocols, performed in random order at the General Clinical Research Center. Each of the protocols will be three days in duration. On the first day of either protocol, a glucose clamp will be performed for measurement of insulin sensitivity. On the second day of the E protocol, baseline parameters of oxidative stress, LDL susceptibility to oxidation and lipid metabolism will be obtained prior to an acute bout of aerobic exercise and periodically throughout the post exercise period, during which three meals will be consumed. On the second day of the NE protocol the same protocol will be followed without the acute exercise bout. On the morning of the third day of each protocol (24 hours after the E or NE session), fasting parameters of CHO, lipid and oxidative stress will be drawn. The diet will follow the National Cholesterol Education Program guidelines with 30% fat as energy, with 10:10:10% from saturated, monounsaturated and polyunsaturated fat, respectively with 1/3 of energy requirements consumed at each meal. This study represent a novel approach to the use of aerobic exercise in the treatment of type 2 DM, providing data for future investigations and exercise training studies in this area.

Agency
National Institute of Health (NIH)
Institute
National Institute of Nursing Research (NINR)
Type
Research Project (R01)
Project #
1R01NR007760-01A2
Application #
6580148
Study Section
Nursing Research Study Section (NURS)
Program Officer
Toward, Jeffrey I
Project Start
2003-09-01
Project End
2006-06-30
Budget Start
2003-09-01
Budget End
2004-06-30
Support Year
1
Fiscal Year
2003
Total Cost
$441,256
Indirect Cost
Name
University of Illinois at Chicago
Department
Other Health Professions
Type
Schools of Nursing
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612
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Farabi, Sarah S; Carley, David W; Smith, Donald et al. (2015) Impact of exercise on diurnal and nocturnal markers of glycaemic variability and oxidative stress in obese individuals with type 2 diabetes or impaired glucose tolerance. Diab Vasc Dis Res 12:381-5
Eren-Oruklu, Meriyan; Cinar, Ali; Quinn, Lauretta et al. (2009) Estimation of future glucose concentrations with subject-specific recursive linear models. Diabetes Technol Ther 11:243-53