This research on myelinogenesis explores the hypothesis that the intracellular processing and assembly of myelin is defective in the quaking mutant. This thesis has been developed by determining the short-term rates of biosynthesis of the major proteins and lipids in the CNS of quaking mutant and the rates of their incorporation into myelin and other subcellular membranes. We shall proceed with further detailed studies of this mutant examining the steps of intracellular protein passage in the process of assemply of the myelin membrane. The main thrust will be to examine the distribution and intracellular processing of myelin protein of two types: intrinsic (the proteolipid protein [PLP] and myelin-associated glycoprotein [MAG]) and peripheral (the four low molecular weight myelin basic proteins [MBPs]). The intracellular processing of myelin proteins subject to post-translational modification in oligodendroglial endomembranes will be examined in quaking CNS (and their analogues in the Schwann cell in PNS) with reference to oligosaccharide processing and acylation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS012044-10
Application #
3394689
Study Section
Neurology A Study Section (NEUA)
Project Start
1976-05-01
Project End
1986-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
10
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Medical University of South Carolina
Department
Type
Schools of Medicine
DUNS #
183710748
City
Charleston
State
SC
Country
United States
Zip Code
29425
Chakrabarti, A K; Banik, N L; Lobo, D C et al. (1993) Calcium-activated neutral proteinase (calpain) in rat brain during development: compartmentation and role in myelination. Brain Res Dev Brain Res 71:107-13
Banik, N L; Chakrabarti, A K; Konat, G W et al. (1992) Calcium-activated neutral proteinase (calpain) activity in C6 cell line: compartmentation of mu and m calpain. J Neurosci Res 31:708-14
Bong, M; Chakrabarti, A; Banik, N et al. (1991) Differential regulation of myelin gene expression in SV40 T antigen-transfected rat glioma C6 cells. Metab Brain Dis 6:7-17
Chakrabarti, A K; Banik, N L; Powers, J M et al. (1989) The regional and subcellular distribution of calcium activated neutral proteinase (CANP) in the bovine central nervous system. Neurochem Res 14:259-66
Hsu, C Y; Halushka, P V; Spicer, K M et al. (1988) Temporal profile of thromboxane-prostacyclin imbalance in experimental spinal cord injury. J Neurol Sci 83:55-62
Chakrabarti, A K; Yoshida, Y; Powers, J M et al. (1988) Calcium-activated neutral proteinase in rat brain myelin and subcellular fractions. J Neurosci Res 20:351-8
Konat, G; Trojanowska, M; Gantt, G et al. (1988) Expression of myelin protein genes in quaking mouse brain. J Neurosci Res 20:19-22
Banik, N L; Happel, R D; Sostek, M B et al. (1987) Ca2+-mediated degradation of central nervous system (CNS) proteins: topographic and species variation. Metab Brain Dis 2:117-26
Banik, N L; Chakrabarti, A K; Gantt, G et al. (1987) Distribution of calcium-activated neutral proteinase activity in quaking mouse brain: a subcellular study. Brain Res 435:57-62
Banik, N L; Chakrabarti, A K; Hogan, E L (1987) Distribution of calcium activated neutral proteinase (mM CANP) in myelin and cytosolic fractions in bovine brain white matter. Life Sci 41:1089-95

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