Recent immunocytochemical and histofluorescent studies have established the existence of serotonergic fibers arising from the raphe complex and projecting to the cerebellar cortex. Ultrastructurally, the indoleaminergic fibers are known to establish synaptic contacts as well as diffuse, meandering nonsynaptic terminations on various cell types, including Purkinje cells. These anatomical findings suggest that serotonin may function as a neurotransmitter within the cerebellum, a suggestion that needs to be examined electrophysiologically at the cellular level. The objectives of the proposed research are (1) to define the changes in spontaneous activity of Purkinje neurons induced by exogenously administered serotonin, (2) to pharmacologically characterize the serotonergic nature of the observed responses, and (3) to determine whether the effects of serotonin invoke pre-and/or postsynaptic sites of action. Methods are described for (1) the microiontophoretic application of substances in a manner to determine dose response relationships, (2) single unit recording of the Purkinje cell and (3) the systematic examination of possible presynaptic sites of serotonin effects that include all known afferent putative transmitters to the Purkinje cell.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS019296-06
Application #
3399318
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1983-04-01
Project End
1992-08-31
Budget Start
1988-09-01
Budget End
1992-08-31
Support Year
6
Fiscal Year
1988
Total Cost
Indirect Cost
Name
Texas Tech University
Department
Type
Schools of Medicine
DUNS #
609980727
City
Lubbock
State
TX
Country
United States
Zip Code
79430
Guo, Wei; Chu, Yu-Xia; Imai, Satoshi et al. (2016) Further observations on the behavioral and neural effects of bone marrow stromal cells in rodent pain models. Mol Pain 12:
Netzeband, J G; Weathers, L B; Strahlendorf, H K et al. (1993) Serotonin depresses excitatory amino acid-induced excitation of cerebellar Purkinje cells in the adult rat in vivo. Brain Res 608:145-9
Cumming-Hood, P A; Strahlendorf, H K; Strahlendorf, J C (1993) Effects of serotonin and the 5-HT2/1C receptor agonist DOI on neurons of the cerebellar dentate/interpositus nuclei: possible involvement of a GABAergic interneuron. Eur J Pharmacol 236:457-65
Li, S J; Wang, Y; Strahlendorf, H K et al. (1993) Serotonin alters an inwardly rectifying current (Ih) in rat cerebellar Purkinje cells under voltage clamp. Brain Res 617:87-95
Wang, Y; Strahlendorf, J C; Strahlendorf, H K (1992) Serotonin reduces a voltage-dependent transient outward potassium current and enhances excitability of cerebellar Purkinje cells. Brain Res 571:345-9
Wang, Y; Strahlendorf, J C; Strahlendorf, H K (1991) A transient voltage-dependent outward potassium current in mammalian cerebellar Purkinje cells. Brain Res 567:153-8
Darrow, E; Strahlendorf, J C; Strahlendorf, H K (1991) Extracellular magnesium concentration alters Purkinje cell responsiveness to serotonin and analogues. Eur J Pharmacol 209:19-25
Strahlendorf, J C; Lee, M H; Strahlendorf, H K (1991) Serotonin modulates muscimol- and baclofen-elicited inhibition of cerebellar Purkinje cells. Eur J Pharmacol 201:239-42
Darrow, E J; Strahlendorf, H K; Strahlendorf, J C (1990) Response of cerebellar Purkinje cells to serotonin and the 5-HT1A agonists 8-OH-DPAT and ipsapirone in vitro. Eur J Pharmacol 175:145-53
Strahlendorf, J C; Lee, M; Strahlendorf, H K (1989) Modulatory role of serotonin on GABA-elicited inhibition of cerebellar Purkinje cells. Neuroscience 30:117-25

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