Abstract

It is proposed to study the role of cyclic AMP, calcium and protein phosphorylation in the peripheral nervous tissue. One approach is to determine the effect of neuronal activity on the state of phosphorylation Protein I in the superior cervical ganglia. A second approach is to determine the site of such phosphorylation in the Protein I molecule. A third approach is to determine the effect of impulse conduction on the state of phosphorylation of Protein I in the vas deferens. A fourth approach is to determine the site of such phosphorylation in the Protein I molecule. A fifth approach is to carry out cytochemical and radioimmunoassay studies, at the light and electromicroscope levels, on the distribution in the peripheral nervous system of Protein X, a protein found in mammalian brain.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS021550-05
Application #
3402751
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Project Start
1984-05-01
Project End
1992-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
5
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Rockefeller University
Department
Type
Graduate Schools
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065

  Publications

Buxbaum, J D; Gandy, S E; Cicchetti, P et al. (1990) Processing of Alzheimer beta/A4 amyloid precursor protein: modulation by agents that regulate protein phosphorylation. Proc Natl Acad Sci U S A 87:6003-6
Hemmings Jr, H C; Nairn, A C; Elliott, J I et al. (1990) Synthetic peptide analogs of DARPP-32 (Mr 32,000 dopamine- and cAMP-regulated phosphoprotein), an inhibitor of protein phosphatase-1. Phosphorylation, dephosphorylation, and inhibitory activity. J Biol Chem 265:20369-76
Thiel, G; Sudhof, T C; Greengard, P (1990) Synapsin II. Mapping of a domain in the NH2-terminal region which binds to small synaptic vesicles. J Biol Chem 265:16527-33
Schalling, M; Djurfeldt, M; Hokfelt, T et al. (1990) Distribution and cellular localization of DARPP-32 mRNA in rat brain. Brain Res Mol Brain Res 7:139-49
Torri-Tarelli, F; Villa, A; Valtorta, F et al. (1990) Redistribution of synaptophysin and synapsin I during alpha-latrotoxin-induced release of neurotransmitter at the neuromuscular junction. J Cell Biol 110:449-59
Navone, F; Di Gioia, G; Jahn, R et al. (1989) Microvesicles of the neurohypophysis are biochemically related to small synaptic vesicles of presynaptic nerve terminals. J Cell Biol 109:3425-33
Hirano, A A; Greengard, P; Huganir, R L (1988) Protein tyrosine kinase activity and its endogenous substrates in rat brain: a subcellular and regional survey. J Neurochem 50:1447-55
Weeks, G; Picciotto, M; Nairn, A C et al. (1988) Purification and characterization of PCPP-260: a Purkinje cell-enriched cyclic AMP-regulated membrane phosphoprotein of Mr 260,000. Synapse 2:89-96
Meister, B; Fried, G; Hokfelt, T et al. (1988) Immunohistochemical evidence for the existence of a dopamine- and cyclic AMP-regulated phosphoprotein (DARPP-32) in brown adipose tissue of pigs. Proc Natl Acad Sci U S A 85:8713-6
Gandy, S; Czernik, A J; Greengard, P (1988) Phosphorylation of Alzheimer disease amyloid precursor peptide by protein kinase C and Ca2+/calmodulin-dependent protein kinase II. Proc Natl Acad Sci U S A 85:6218-21

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