Abstract

Detailed information concerning the prenatal neurogenesis of some neurons and fibers of the human cerebellum is lacking. I propose to carry out prenatal developmental studies of various neurons and fibers of the human cerebellum and to analyze their developmental interrelationships. I will use the rapid Golgi method in these developmental studies. The principal objectives to pursue are the following. (1) Neurogenesis of the mossy fibers, and of its multiple neuronal connections. This study will also try to determine if the human climbing and mossy fibers are indistinguishable, early in their development and share temporarily the same neuronal targets. (2) Neurogenesis of the Golgi cell. This study will try to determine if there are more than one type of Golgi cell in the human cerebellum, or, if they have more than one maturation spur. (3) Neurogenesis of the Lugaro and Interstitial cells. This study will try to determine if these neurons are structurally and functionally part of the basic Climbing-Purkinje System. (4) Neurogenesis of the abnormal cerebellum of four infants with bilateral renal agenesis (Potter's syndrome). Preliminary observations have disclosed peculiar dendritic and axonic anomalies in the structure of developing Purkinje cells not previously described. (5) Progressive reconstruction of 'maps' of the fibrillar neuronal organization of the human cerebellum at different prenatal (25, 30, 35, and 40 week gestation) and early postnatal (1 and 5 year of age) developmental stages, to be used in future studies of normal and abnormal cerebellar development. (6) Continue by Golgi studies of the human cerebral cortex which started many years ago.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS022897-02
Application #
3405681
Study Section
Neurology B Subcommittee 1 (NEUB)
Project Start
1986-02-01
Project End
1989-01-31
Budget Start
1987-02-01
Budget End
1988-01-31
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Dartmouth College
Department
Type
Schools of Medicine
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755

  Publications

Marin-Padilla, Miguel; Parisi, Joseph E; Armstrong, Dawna L et al. (2002) Shaken infant syndrome: developmental neuropathology, progressive cortical dysplasia, and epilepsy. Acta Neuropathol (Berl) 103:321-32
Marin-Padilla, M (2000) Perinatal brain damage, cortical reorganization (acquired cortical dysplasias), and epilepsy. Adv Neurol 84:153-72
Marin-Padilla, M (1999) Developmental neuropathology and impact of perinatal brain damage. III: gray matter lesions of the neocortex. J Neuropathol Exp Neurol 58:407-29
North, T; Gu, T L; Stacy, T et al. (1999) Cbfa2 is required for the formation of intra-aortic hematopoietic clusters. Development 126:2563-75
Marin-Padilla, M (1999) [The development of the human cerebral cortex. A cytoarchitectonic theory] Rev Neurol 29:208-16
Marin-Padilla, M (1998) Cajal-Retzius cells and the development of the neocortex. Trends Neurosci 21:64-71
Marin-Padilla, M (1997) Developmental neuropathology and impact of perinatal brain damage. II: white matter lesions of the neocortex. J Neuropathol Exp Neurol 56:219-35
Marin-Padilla, M (1997) [Pathology and pathogenesis of secondary epilepsy to hypoxic-ischemic encephalopathies] Rev Neurol 25:673-82
Yergeau, D A; Hetherington, C J; Wang, Q et al. (1997) Embryonic lethality and impairment of haematopoiesis in mice heterozygous for an AML1-ETO fusion gene. Nat Genet 15:303-6
Wang, Q; Stacy, T; Binder, M et al. (1996) Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. Proc Natl Acad Sci U S A 93:3444-9

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