Abstract

We will be developing a lineage map of the chick retina using a new technology based on retrovirus vectors. The retrovirus vectors are ideal markers for lineage mapping in that they reproducibly integrate into virtually any cell type in most mammalian and avian species. This integration feature allows for the marking of a cell and all of its progeny. We will construct vectors which encode histochemical markers in order to provide a facile means to identify infected cells in tissue sections, and develop a methodology for infecting and assaying chick embryos. The chick retina will be the primary organ which will be analyzed using this new technique. The retina is well described anatomically and functionally and should provide for easy access in both early and late development stages. We wish to assess the lineage relationships among the various neuronal and glial cell types within the retina. We will also investigate questions of lineage within the retinal ganglion cells of the retina in order to assess the role of lineage in the establishment of the retino-tectal map. If we are successful in these studies, the vectors and methods described here will allow for lineage mapping in virtually any tissue in most mammalian and avian species.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS023021-03
Application #
3405972
Study Section
Neurology C Study Section (NEUC)
Project Start
1986-08-01
Project End
1989-07-31
Budget Start
1988-08-01
Budget End
1989-07-31
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Golden, J A; Cepko, C L (1996) Clones in the chick diencephalon contain multiple cell types and siblings are widely dispersed. Development 122:65-78
Arnold-Aldea, S A; Cepko, C L (1996) Dispersion patterns of clonally related cells during development of the hypothalamus. Dev Biol 173:148-61
Golden, J A; Fields-Berry, S C; Cepko, C L (1995) Construction and characterization of a highly complex retroviral library for lineage analysis. Proc Natl Acad Sci U S A 92:5704-8
Fekete, D M; Perez-Miguelsanz, J; Ryder, E F et al. (1994) Clonal analysis in the chicken retina reveals tangential dispersion of clonally related cells. Dev Biol 166:666-82
Ryder, E F; Cepko, C L (1994) Migration patterns of clonally related granule cells and their progenitors in the developing chick cerebellum. Neuron 12:1011-28
Sucher, N J; Brose, N; Deitcher, D L et al. (1993) Expression of endogenous NMDAR1 transcripts without receptor protein suggests post-transcriptional control in PC12 cells. J Biol Chem 268:22299-304
Walsh, C; Cepko, C L (1992) Widespread dispersion of neuronal clones across functional regions of the cerebral cortex. Science 255:434-40
Halliday, A L; Cepko, C L (1992) Generation and migration of cells in the developing striatum. Neuron 9:15-26
Fields-Berry, S C; Halliday, A L; Cepko, C L (1992) A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc Natl Acad Sci U S A 89:693-7
Misson, J P; Austin, C P; Takahashi, T et al. (1991) The alignment of migrating neural cells in relation to the murine neopallial radial glial fiber system. Cereb Cortex 1:221-9

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