Fundamental parameters of cortical and cerebellar development will be Studied using retrovirus-mediated gene transfer. Through introduction of a histochemical marker gene carried by a retrovirus vector, cells will be tagged with an innocuous and stable genetic marker, enabling an analysis of fate within the developing brain of rats and mice. Patterns of proliferation, migration and lineage will be examined. The role of lineage in the determination of cytoarchitectonic boundaries will also be investigated. The protocols employed in this proposal will build upon the previous methodology developed in the prior grant period. Improvements to the existing technology will be made by way of the development of novel histochemical marker viruses.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS023021-04
Application #
3405970
Study Section
Neurology C Study Section (NEUC)
Project Start
1986-08-01
Project End
1994-07-31
Budget Start
1989-08-01
Budget End
1990-07-31
Support Year
4
Fiscal Year
1989
Total Cost
Indirect Cost
Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115
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Halliday, A L; Cepko, C L (1992) Generation and migration of cells in the developing striatum. Neuron 9:15-26
Fields-Berry, S C; Halliday, A L; Cepko, C L (1992) A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc Natl Acad Sci U S A 89:693-7
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