Abstract

Fundamental parameters of cortical and cerebellar development will be Studied using retrovirus-mediated gene transfer. Through introduction of a histochemical marker gene carried by a retrovirus vector, cells will be tagged with an innocuous and stable genetic marker, enabling an analysis of fate within the developing brain of rats and mice. Patterns of proliferation, migration and lineage will be examined. The role of lineage in the determination of cytoarchitectonic boundaries will also be investigated. The protocols employed in this proposal will build upon the previous methodology developed in the prior grant period. Improvements to the existing technology will be made by way of the development of novel histochemical marker viruses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
2R01NS023021-04
Application #
3405970
Study Section
Neurology C Study Section (NEUC)
Project Start
1986-08-01
Project End
1994-07-31
Budget Start
1989-08-01
Budget End
1990-07-31
Support Year
4
Fiscal Year
1989
Total Cost
Indirect Cost

  Institution

Name
Harvard University
Department
Type
Schools of Medicine
DUNS #
082359691
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Golden, J A; Cepko, C L (1996) Clones in the chick diencephalon contain multiple cell types and siblings are widely dispersed. Development 122:65-78
Arnold-Aldea, S A; Cepko, C L (1996) Dispersion patterns of clonally related cells during development of the hypothalamus. Dev Biol 173:148-61
Golden, J A; Fields-Berry, S C; Cepko, C L (1995) Construction and characterization of a highly complex retroviral library for lineage analysis. Proc Natl Acad Sci U S A 92:5704-8
Fekete, D M; Perez-Miguelsanz, J; Ryder, E F et al. (1994) Clonal analysis in the chicken retina reveals tangential dispersion of clonally related cells. Dev Biol 166:666-82
Ryder, E F; Cepko, C L (1994) Migration patterns of clonally related granule cells and their progenitors in the developing chick cerebellum. Neuron 12:1011-28
Sucher, N J; Brose, N; Deitcher, D L et al. (1993) Expression of endogenous NMDAR1 transcripts without receptor protein suggests post-transcriptional control in PC12 cells. J Biol Chem 268:22299-304
Walsh, C; Cepko, C L (1992) Widespread dispersion of neuronal clones across functional regions of the cerebral cortex. Science 255:434-40
Halliday, A L; Cepko, C L (1992) Generation and migration of cells in the developing striatum. Neuron 9:15-26
Fields-Berry, S C; Halliday, A L; Cepko, C L (1992) A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc Natl Acad Sci U S A 89:693-7
Misson, J P; Austin, C P; Takahashi, T et al. (1991) The alignment of migrating neural cells in relation to the murine neopallial radial glial fiber system. Cereb Cortex 1:221-9

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