Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
1R01NS034348-01A1
Application #
2273550
Study Section
Neurological Sciences Subcommittee 1 (NLS)
Program Officer
Kitt, Cheryl A
Project Start
1996-05-01
Project End
1999-04-30
Budget Start
1996-05-01
Budget End
1997-04-30
Support Year
1
Fiscal Year
1996
Total Cost
Indirect Cost

  Institution

Name
Brown University
Department
Pharmacology
Type
Schools of Medicine
DUNS #
001785542
City
Providence
State
RI
Country
United States
Zip Code
02912

  Publications

Sanders, Tanya; Hawrot, Edward (2004) A novel pharmatope tag inserted into the beta4 subunit confers allosteric modulation to neuronal nicotinic receptors. J Biol Chem 279:51460-5
Zeng, Haoyu; Hawrot, Edward (2002) NMR-based binding screen and structural analysis of the complex formed between alpha-cobratoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica. J Biol Chem 277:37439-45
Moise, Leonard; Piserchio, Andrea; Basus, Vladimir J et al. (2002) NMR structural analysis of alpha-bungarotoxin and its complex with the principal alpha-neurotoxin-binding sequence on the alpha 7 subunit of a neuronal nicotinic acetylcholine receptor. J Biol Chem 277:12406-17
Zeng, H; Moise, L; Grant, M A et al. (2001) The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica. J Biol Chem 276:22930-40
Levandoski, M M; Caffery, P M; Rogowski, R S et al. (2000) Recombinant expression of alpha-bungarotoxin in Pichia pastoris facilitates identification of mutant toxins engineered to recognize neuronal nicotinic acetylcholine receptors. J Neurochem 74:1279-89
Grant, M A; Gentile, L N; Shi, Q L et al. (1999) Expression and spectroscopic analysis of soluble nicotinic acetylcholine receptor fragments derived from the extracellular domain of the alpha-subunit. Biochemistry 38:10730-42
Levandoski, M M; Lin, Y; Moise, L et al. (1999) Chimeric analysis of a neuronal nicotinic acetylcholine receptor reveals amino acids conferring sensitivity to alpha-bungarotoxin. J Biol Chem 274:26113-9
Rosenthal, J A; Levandoski, M M; Chang, B et al. (1999) The functional role of positively charged amino acid side chains in alpha-bungarotoxin revealed by site-directed mutagenesis of a His-tagged recombinant alpha-bungarotoxin. Biochemistry 38:7847-55
Hawrot, E; Xiao, Y; Shi, Q L et al. (1998) Demonstration of a tandem pair of complement protein modules in GABA(B) receptor 1a. FEBS Lett 432:103-8