The mammalian neocortex is an enormous network of cells, each making thousands of connections and an array of neurological conditions can result from inappropriate cortical structure or connectivity. Many of these congenital brain defects have a genetic origin but we still lack a full understanding of the genes and mechanisms involved. The overall objective of this application is to use forward genetic approaches in mouse and human to identify and validate novel alleles important for development of cortical circuitry and overall structure. Our central hypothesis is that a synergistic and unbiased forward genetic approach in mouse and human will lead to fundamental discoveries in the genetics of cortical circuit formation and structural development. The rationale of this proposed research is that by identifying novel genes through forward genetic approaches which are required for normal cortical development using both human and mouse genetics, we are then positioned to use this information and tools to study the etiological mechanisms of human cortical malformations in subsequent studies. We will test this central hypothesis and accomplish the goals of this application by pursuing the following three specific aims: 1) use forward genetics in the mouse to efficiently generate and capture genetic mutations in loci important for cortical circuit formation and structural development, 2) identify and validate causal mutations in novel mouse models of cortical circuit formation and structural brain defects, and 3) apply next-generation sequencing approaches to identify mutations leading to human movement disorders and structural brain defects.
The aims are accomplished by an ENU mutagenesis approach in the mouse with the addition of a novel transgenic reporter which is expressed specifically in cortical layer V pyramidal neurons. The mutations are then cloned and validated through a number of functional studies. The human genetics studies are performed with the application of exome sequencing to carefully selected familial cases of movement disorders and structural brain malformations. These studies will identify several genes essential for mammalian forebrain structure and function. The significance of this work is found in the specific application to cortial circuitry and structure, and that an unbiased approach such as this has the capability to implicate entirely new pathways in neurological disease. A synergistic approach using both mouse and human genetics to specifically query these aspects of neural development allows fundamental insights into the genetics of development and disease. Such knowledge is not only critical to further understand the basic mechanisms of neurodevelopment, but also has immediate clinical relevance through identification of a number of potential therapeutic targets. Furthermore, these mouse models provide a reusable resource to directly characterize the role of the mutated gene in neurodevelopment, and potentially serve as a tool to test future therapeutic interventions. Taken together, these findings are therefore applicable to basic developmental neurobiology, pediatric and adult neurology, human genetics and genetic counseling.

Public Health Relevance

Birth defects affecting the structure of the mammalian nervous system are fairly common (~15/10,000 births). While these congenital defects have a genetic component, the precise genes responsible are still largely unknown. This proposal will use genetic approaches with emerging sequencing and animal transgenic technologies to study the etiology of defects in forebrain circuit formation and function. These studies will lead to mor effective clinical genetics, diagnostics, and therapeutic interventions.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Research Project (R01)
Project #
Application #
Study Section
Genetics of Health and Disease Study Section (GHD)
Program Officer
Riddle, Robert D
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
United States
Zip Code
Abdelhamed, Zakia; Vuong, Shawn M; Hill, Lauren et al. (2018) A mutation in Ccdc39 causes neonatal hydrocephalus with abnormal motile cilia development in mice. Development 145:
Li, Zhuo; Peng, Yanyan; Hufnagel, Robert B et al. (2017) Loss of SLC25A46 causes neurodegeneration by affecting mitochondrial dynamics and energy production in mice. Hum Mol Genet 26:3776-3791
Stottmann, Rolf W; Driver, Ashley; Gutierrez, Arnold et al. (2017) A heterozygous mutation in tubulin, beta 2B ( Tubb2b ) causes cognitive deficits and hippocampal disorganization. Genes Brain Behav 16:250-259
DiStasio, Andrew; Driver, Ashley; Sund, Kristen et al. (2017) Copb2 is essential for embryogenesis and hypomorphic mutations cause human microcephaly. Hum Mol Genet 26:4836-4848
Pedapati, Ernest V; Gilbert, Donald L; Erickson, Craig A et al. (2016) Abnormal Cortical Plasticity in Youth with Autism Spectrum Disorder: A Transcranial Magnetic Stimulation Case-Control Pilot Study. J Child Adolesc Psychopharmacol 26:625-31
Go, D E; Stottmann, R W (2016) The Impact of CRISPR/Cas9-Based Genomic Engineering on Biomedical Research and Medicine. Curr Mol Med 16:343-52
Cionni, Megan; Menke, Chelsea; Stottmann, Rolf W (2016) Novel genetic tools facilitate the study of cortical neuron migration. Mamm Genome 27:8-16
Gelineau-Morel, Rose; Lukacs, Marshall; Weaver, K Nicole et al. (2016) Congenital Cataracts and Gut Dysmotility in a DYNC1H1 Dyneinopathy Patient. Genes (Basel) 7:
Driver, Ashley M; Kratz, Lisa E; Kelley, Richard I et al. (2016) Altered cholesterol biosynthesis causes precocious neurogenesis in the developing mouse forebrain. Neurobiol Dis 91:69-82
Menke, Chelsea; Cionni, Megan; Siggers, Trevor et al. (2015) Grhl2 is required in nonneural tissues for neural progenitor survival and forebrain development. Genesis 53:573-582

Showing the most recent 10 out of 11 publications