Chronic pain caused by injury to the peripheral or central nervous system (neuropathic pain) is notoriously resistant to treatment. The mechanisms that maintain any type of neuropathic pain for months or longer are poorly understood. Chronic pain in a rat model of spinal cord injury (SCI) has recently been shown to depend upon hyperactivity in nociceptive sensory neurons (nociceptors), with much of the pain-initiating activity generated within the cell bodies. The continued expression of pain-linked nociceptor hyper excitability and spontaneous activity (SA) in vitro provides a special opportunity to link biochemical mechanisms directly to electrophysiological activity critical for maintaining chronic SCI pain. Preliminary results indicate that continuing signaling by complexes containing adenylyl cyclase (AC), protein kinase A (PKA), and A-kinase anchoring proteins (AKAPs), and possibly exchange protein activated by cAMP (EPAC) plays a vital role. While cAMP signaling has long been known to be important for acute pain lasting hours to days, a major role in maintaining pain lasting months is unexpected. Agents selectively inhibiting different steps along cAMP-dependent pathways blocked chronic SCI-induced SA, including inhibitors of AKAP5 (AKAP79/150)-anchored complexes. Biochemical studies of membranes from dorsal root ganglia revealed a change in AC regulation after SCI, suggesting the existence of a previously unknown mechanism at the level of AC function that contributes to chronic pain. These and related observations led to the hypothesis that chronic nociceptor SA and pain after SCI are maintained by 1) alterations in AC regulation and 2) AKAP5-scaffolded macromolecular complexes that facilitate cAMP-dependent PKA and EPAC regulation of ion channels. The proposed studies will exploit complementary strengths of the two PIs' laboratories by combining in vitro biochemistry, cell biology, and electrophysiology coordinated with in vivo tests of pain-related behavior after SCI. Experiments will take advantage of our findings that robust SCI-induced SA in numerous nociceptors below the spinal injury level is clearly linked to behaviorally expressed hypersensitivity and pain. This will allow the use of electrophysiological and molecular alterations in dissociated nociceptors as informative endpoints for studies evaluating pain-related functions of signaling molecules within the cAMP pathway. It will also allow pooling of multiple ganglia from SCI animals to facilitate biochemical and molecular studies. Predicted behavioral and cellular effects of interventions targeting macromolecular complexes disclosed in the in vitro studies will be tested in the whole animal using complementary approaches, including a novel viral vector for expression of disrupting peptides selectively in nociceptors, an knockdown and inhibitor methods targeting specific cAMP signaling components. Information gained from these studies may lead to major mechanistic discoveries that could guide future efforts to treat chronic pain by targeting the persistent intracellular signaling that maintains hyperactivity in nociceptors that promotes chronic pain.

Public Health Relevance

For many spinal cord injury (SCI) patients, their number one complaint is not confinement to a wheel chair or the inability to walk, but rather is pain. Chronic pain of all kinds takes a staggering toll on the American populace, affecting at least 116 million adults and costing up to $635 billion annually in treatment and lost productivity. Although chronic pain caused by spinal cord injury (SCI) represents a small fraction of this total, at least half of all SCI patients endure life-long pain that resists available treatments. The mechanisms that maintain any form of chronic pain (lasting months to a lifetime) remain mysterious, which helps explain the limited effectiveness of current treatments for chronic pain. This joint project will define mechanistic changes that occur in sensory neurons that contribute to chronic pain after spinal cord injury. It will follow up on the discovery that spinal cord injury causes a large number of these sensory neurons near and below the site of injury to change from their normal state of electrical silence into a persistent state of spontaneous activity. Preliminary data shows that agents that target cyclic AMP signaling pathways decrease spontaneous activity and thus offer future promise for treating this and other forms of chronic pain.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Project (R01)
Project #
5R01NS091759-02
Application #
9124956
Study Section
Molecular and Integrative Signal Transduction Study Section (MIST)
Program Officer
Oshinsky, Michael L
Project Start
2015-08-15
Project End
2020-07-31
Budget Start
2016-08-01
Budget End
2017-07-31
Support Year
2
Fiscal Year
2016
Total Cost
Indirect Cost
Name
University of Texas Health Science Center Houston
Department
Biology
Type
Schools of Medicine
DUNS #
800771594
City
Houston
State
TX
Country
United States
Zip Code
77225
Ferré, Sergi; Bonaventura, Jordi; Zhu, Wendy et al. (2018) Essential Control of the Function of the Striatopallidal Neuron by Pre-coupled Complexes of Adenosine A2A-Dopamine D2 Receptor Heterotetramers and Adenylyl Cyclase. Front Pharmacol 9:243
Walters, Edgar T (2018) Nociceptive Biology of Molluscs and Arthropods: Evolutionary Clues About Functions and Mechanisms Potentially Related to Pain. Front Physiol 9:1049
Luo, Jialie; Bavencoffe, Alexis; Yang, Pu et al. (2018) Zinc Inhibits TRPV1 to Alleviate Chemotherapy-Induced Neuropathic Pain. J Neurosci 38:474-483
Walters, Edgar T (2018) How is chronic pain related to sympathetic dysfunction and autonomic dysreflexia following spinal cord injury? Auton Neurosci 209:79-89
Odem, Max A; Bavencoffe, Alexis G; Cassidy, Ryan M et al. (2018) Isolated nociceptors reveal multiple specializations for generating irregular ongoing activity associated with ongoing pain. Pain 159:2347-2362
Figueroa, Javier; Phillips, Lynette M; Shahar, Tal et al. (2017) Exosomes from Glioma-Associated Mesenchymal Stem Cells Increase the Tumorigenicity of Glioma Stem-like Cells via Transfer of miR-1587. Cancer Res 77:5808-5819
Dessauer, Carmen W; Watts, Val J; Ostrom, Rennolds S et al. (2017) International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases. Pharmacol Rev 69:93-139
Dessauer, Carmen W (2017) Shining a light on GPCR complexes. J Biol Chem 292:14290-14291
Cuevas-Diaz Duran, Raquel; Wei, Haichao; Wu, Jia Qian (2017) Single-cell RNA-sequencing of the brain. Clin Transl Med 6:20
Bavencoffe, Alexis; Li, Yong; Wu, Zizhen et al. (2016) Persistent Electrical Activity in Primary Nociceptors after Spinal Cord Injury Is Maintained by Scaffolded Adenylyl Cyclase and Protein Kinase A and Is Associated with Altered Adenylyl Cyclase Regulation. J Neurosci 36:1660-8