The overall objective of this project is to better understand how apoptosis occurs in normal breast cells in order to understand how breast cancer can be prevented. This proposal aims to characterize apoptosis in human MCF-10A breast cells, which are immortalized, non-tumorigenic, non-estrogen-responsive cells.
Specific aims are to:1. Determine the mechanism of apoptosis following growth-factor withdrawalin MCF-10A breast epithelial cells, and specifically determine to what extent mitochondria are involved in apoptosis in these cells. 2. Determine how the mechanism of apoptosis followinggrowth-factor-withdrawal is altered in MCF-10A cells that stably express Bcl-2. 3. Determine the pattern of mitochondrial gene expression during apoptosis in MCF-10A and MCF-10A/Bcl-2 cells.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
1R15CA087810-01
Application #
6190655
Study Section
Metabolic Pathology Study Section (MEP)
Program Officer
Spalholz, Barbara A
Project Start
2000-09-01
Project End
2003-08-31
Budget Start
2000-09-01
Budget End
2003-08-31
Support Year
1
Fiscal Year
2000
Total Cost
$139,500
Indirect Cost
Name
East Carolina University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Greenville
State
NC
Country
United States
Zip Code
27858