In aim 1, a 3 x 7 synthetic matrix of substituted N-phenyl-arenecarboximides will be used for screening and identifying dual fluorescent dyes. The proposed matrix elements are based on preliminary findings which indicate optimum substituent placement on arene components to promote the unique dual fluorescent phenomena. To demonstrate the versatility of these 2-color systems, functional groups for various ionic and neutral analytes are briefly outlined.
Aim 2 of the application involves the use of the """"""""Internal Stark Effect"""""""" as a minimal modification approach to extending the absorption and emission wavelengths of these fluorescent probes. Their electrochromic behavior would allow for direct application as fluorescent probes for measuring micropolarity in surfaces of natural and synthetic macromolecules, micelles, phospholipid bilayers, and biomembranes.
Aim 3 projections for this work include the development of arenedicarboximides as fluorescent proteomimetics for potential use in mediating protein-protein interactions. These probes would allow for direct optical measurement of helix surface recognition and therefore providing binding information along targeted sites of the protein. ? ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Academic Research Enhancement Awards (AREA) (R15)
Project #
2R15GM057855-03
Application #
7071469
Study Section
Special Emphasis Panel (ZRG1-BCMB-R (90))
Program Officer
Deatherage, James F
Project Start
1998-09-01
Project End
2009-03-31
Budget Start
2006-04-01
Budget End
2009-03-31
Support Year
3
Fiscal Year
2006
Total Cost
$214,820
Indirect Cost
Name
New Mexico Institute of Mining & Technology
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
041358904
City
Socorro
State
NM
Country
United States
Zip Code
87801
Paudel, Sangita; Nandhikonda, Premchendar; Heagy, Michael D (2009) A comparative study into two dual fluorescent mechanisms via positional isomers of N-hydroxyarene-1,8-naphthalimides. J Fluoresc 19:681-91
Cao, Zhi; Nandhikonda, Premchendar; Heagy, Michael D (2009) Highly water-soluble monoboronic acid probes that show optical sensitivity to glucose based on 4-sulfo-1,8-naphthalic anhydride. J Org Chem 74:3544-6
Cao, Haishi; McGill, Tom; Heagy, Michael D (2004) Substituent effects on monoboronic acid sensors for saccharides based on N-phenyl-1,8-naphthalenedicarboximides. J Org Chem 69:2959-66