Hepatocellular carcinoma (HCC) is the fifth most common cancer world-wide, contributing to one third of all cancer-related deaths. Current treatment options for HCC include surgical resection, chemo- and radioembolization, transplantation, and stereotactic radiosurgery. The only approved pharmacological interventions for advanced disease include Sorafenib, a multi-tyrosine kinase inhibitor, in combination with doxorubicin. However, this treatment regime only extends recurrence-free survival by several months. In most cases, patients will succumb to the disease within a year following diagnosis. This highlights an urgent need to identify new therapeutic targets in HCC. Considerable evidence supports a role for de novo lipogenesis (DNL) in HCC development. However, this process has never been targeted in vivo in liver cancer. Herein we propose two Specific Aims that will evaluate the role of DNL in HCC initiation and progression.
Aim 1 will test ethanol-induced tumor initiation and progression following carcinogen treatment (diethylnitrosamine) in a novel line of transgenic mice that lack hepatic expression of the rate limiting lipogenic enzymes acetyl-CoA carboxylases (ACC) 1 and 2.
Aim 2 will evaluate how lipogenic nutrients in the diet contribute to HCC progression when combined with ethanol exposure. In this Aim we will also test the efficacy of a novel orally bioavailable ACC inhibitor to reduce tumor burden. Minimally, this project will determine whether ACC enzymes represent a pharmacological target for the treatment of HCC both with and without an ethanol stimulus. Maximally, we will identify ACC enzymes as new drug targets that can be pharmacologically inhibited in vivo to reduce liver tumor burden.
Primary liver cancer is one of the most lethal and incurable malignancies worldwide and ethanol is thought to contribute to up to one-third of cases. In this study we will test the hypothesis that ethanol consumption drives the production of lipids within the liver to facilitate tumor initiation and progression. We will test this in mouse models lacking hepatic lipogenesis and in mice fed diets that differ in lipogenic capacity.
|Nelson, Marin E; Lahiri, Sujoy; Chow, Jenny D Y et al. (2017) Inhibition of hepatic lipogenesis enhances liver tumorigenesis by increasing antioxidant defence and promoting cell survival. Nat Commun 8:14689|
|Taddeo, Evan P; Hargett, Stefan R; Lahiri, Sujoy et al. (2017) Lysophosphatidic acid counteracts glucagon-induced hepatocyte glucose production via STAT3. Sci Rep 7:127|
|Brandon, Amanda E; Stuart, Ella; Leslie, Simon J et al. (2016) Minimal impact of age and housing temperature on the metabolic phenotype of Acc2-/- mice. J Endocrinol 228:127-34|
|Byrne, Frances L; Hoehn, Kyle L (2016) Subclassification of fatty liver by its pathogenesis: cIEFing is believing. J Pathol 239:3-5|
|Healy, Marin E; Lahiri, Sujoy; Hargett, Stefan R et al. (2016) Dietary sugar intake increases liver tumor incidence in female mice. Sci Rep 6:22292|
|Healy, Marin E; Chow, Jenny D Y; Byrne, Frances L et al. (2015) Dietary effects on liver tumor burden in mice treated with the hepatocellular carcinogen diethylnitrosamine. J Hepatol 62:599-606|
|Kashatus, Jennifer A; Nascimento, Aldo; Myers, Lindsey J et al. (2015) Erk2 phosphorylation of Drp1 promotes mitochondrial fission and MAPK-driven tumor growth. Mol Cell 57:537-51|