Abstract

The investigator proposes to study the phenotype and function of fine T cell subsets in normal adults, neonatal and pediatric samples, BMT/stem cell transplant patients, chemotherapy/irradiation treated patients, and HIV-infected patients before and after effective anti- viral therapy. The flow cytometry technology available in this lab allows the simultaneous 10-color, 1 parameter analysis of cells that can more clearly identify subsets of T cells and allow the unraveling of the considerable confusion and controversy surrounding the AIDS immunophenotyping literature.
The aims are to determine the parameter that define functionally important T cell subsets, define the functional capacity of the subsets, and study the dynamic equilibrium of the subsets in clinically relevant populations. The data obtained will potentially be useful for elucidating the functional capacity of rare T cell subsets and for the monitoring of T cell reconstitution during anti-viral therapy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21CA081543-02
Application #
2896790
Study Section
Special Emphasis Panel (ZRG5-AARR-2 (03))
Program Officer
Finerty, John F
Project Start
1998-09-30
Project End
2000-09-29
Budget Start
1999-09-30
Budget End
2000-09-29
Support Year
2
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Genetics
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Robert, Guillaume; Gastaldi, Cecile; Puissant, Alexandre et al. (2012) The anti-apoptotic Bcl-B protein inhibits BECN1-dependent autophagic cell death. Autophagy 8:637-49
Fong, Lawrence; Mengozzi, Manuela; Abbey, Nancy W et al. (2002) Productive infection of plasmacytoid dendritic cells with human immunodeficiency virus type 1 is triggered by CD40 ligation. J Virol 76:11033-41
De Rosa, S C; Mitra, D K; Watanabe, N et al. (2001) Vdelta1 and Vdelta2 gammadelta T cells express distinct surface markers and might be developmentally distinct lineages. J Leukoc Biol 70:518-26
De Rosa, S C; Herzenberg, L A; Herzenberg, L A et al. (2001) 11-color, 13-parameter flow cytometry: identification of human naive T cells by phenotype, function, and T-cell receptor diversity. Nat Med 7:245-8
Tjioe, I; Legerton, T; Wegstein, J et al. (2001) Phycoerythrin-allophycocyanin: a resonance energy transfer fluorochrome for immunofluorescence. Cytometry 44:24-9
Nakamura, H; Herzenberg, L A; Bai, J et al. (2001) Circulating thioredoxin suppresses lipopolysaccharide-induced neutrophil chemotaxis. Proc Natl Acad Sci U S A 98:15143-8
Mengozzi, M; Malipatlolla, M; De Rosa, S C et al. (2001) Naive CD4 T cells inhibit CD28-costimulated R5 HIV replication in memory CD4 T cells. Proc Natl Acad Sci U S A 98:11644-9
De Rosa, S C; Zaretsky, M D; Dubs, J G et al. (2000) N-acetylcysteine replenishes glutathione in HIV infection. Eur J Clin Invest 30:915-29
Lee, P P; Yee, C; Savage, P A et al. (1999) Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients. Nat Med 5:677-85