Electronic BioSciences (EBS) will investigate and develop methodologies to accurately sequence microsatellite regions within the human genome to enable cancer genotyping. Microsatellites are simple/short repeats (1-10 nucleotides in length) that occur in tandem 5-50 times, and are among the most variable types of DNA sequence in the genome. Mutations to these microsatellite regions include expansion or contraction of the repeat number, single nucleotide polymorphisms (SNPs), and/or insertions or deletions (indels), which have been documented with cancer predisposition, cancer onset, and/or prognosis for many types of cancer, including colon, breast, bladder, brain, head and neck, skin, thyroid, kidney, prostate, lung, ovarian, and liver. To date, however, there is no technology presently available that is ideally suited for microsatellite sequencing/characterization, which has significantly limited the understanding of microsatellite mutations and the development of associated diagnostic/prognostic assays. During this two-year program, EBS will focus on developing the methodology to sequence and genotype microsatellites from colon cancer cell lines via direct, electronic, nanopore-based, single-molecule sequencing. The investigations performed during this program will enable new approaches to probe the human genome with unprecedented detail in order to relate and statistically characterize microsatellite sequences that signify a specific cancer type, predisposition, onset, and/or prognosis, directly improving basic cancer research and enabling eventual improved clinical diagnostics and/or prognostics technologies.

Public Health Relevance

This program is aimed at developing direct, electronic, nanopore-based, single-molecule sequencing methodologies for microsatellite sequencing to enable cancer genotyping. The investigations performed during this program will lead to improved basic cancer research, and eventually the development of improved clinical diagnostics and/or prognostics technologies. By improving the understanding of cancer-driving mutations and general knowledge of the genome, human health and patient care will be greatly improved.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21CA228948-02
Application #
9756344
Study Section
Special Emphasis Panel (ZCA1)
Program Officer
Li, Jerry
Project Start
2018-08-06
Project End
2020-07-31
Budget Start
2019-08-01
Budget End
2020-07-31
Support Year
2
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Electronic Biosciences, Inc.
Department
Type
DUNS #
129852864
City
San Diego
State
CA
Country
United States
Zip Code
92121