This application seeks to develop a """"""""multi-metal chip"""""""" that will facilitate rapid and accurate point- of-care measurement of blood metal concentration, including manganese (Mn), zinc (Zn), cadmium (Cd), and lead (Pb) in children. Whole blood analysis is the most common method for determining metal exposure and assessment of health outcomes, yet sample collection from children poses multiple challenges, including parent consent and child assent, and the ability to give blood (especially in studies that require repeated measurements). This application aims to transform the current paradigm by demonstrating a """"""""laboratory-in-a-chip"""""""" sensor capable of rapid analysis (5-10min) on small blood samples (2 drops from a single lancet puncture). We propose to develop this system for highly sensitive measurement of Mn, Zn, Cd, and Pb in whole blood by accomplishing the following specific aims: 1) develop a lab-on-a-chip sensor for a rapid, point-of-care multi-metal analysis in whole blood, and 2) conduct a field test and determine the applicability of the multi-metal sensor for measuring blood metal concentration in children residing near a ferromanganese refinery. Our approach will be to integrate anodic stripping voltammetry with our deep expertise in lab-on-a-chip and microfluidics. Our multidisciplinary team of engineers, chemists, and environmental health has taken the first steps towards developing such a device. We have demonstrated feasibility of the sensor through pilot tests, and are now poised to fully develop the sensor system for point-of-care application. The uniqueness of this study is the child-friendly requirement of only drops of blood for analysis, low- cost (disposable), ability to conduct multiple blood measurements, and its potential use in large- scale clinic, occupational and research settings, such as the National Children's Study and the National Health and Nutrition Examination Survey.

Public Health Relevance

This application seeks to develop a """"""""laboratory-in-a-chip"""""""" sensor for rapid and accurate point-of- care measurement of metals (manganese, zinc, cadmium, and lead) blood. Whole blood analysis is the most common method for determining metal exposure and assessment of health outcomes, yet sample collection from children is highly problematic. The uniqueness of this study is the child-friendly requirement of only drops of blood for analysis, low-cost, ability to conduct multiple blood measurements, and potential use in large-scale clinic, occupational and research settings.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Exploratory/Developmental Grants (R21)
Project #
5R21ES019255-02
Application #
8136560
Study Section
Instrumentation and Systems Development Study Section (ISD)
Program Officer
Balshaw, David M
Project Start
2010-09-01
Project End
2013-08-31
Budget Start
2011-09-01
Budget End
2013-08-31
Support Year
2
Fiscal Year
2011
Total Cost
$229,759
Indirect Cost
Name
University of Cincinnati
Department
Engineering (All Types)
Type
Schools of Engineering
DUNS #
041064767
City
Cincinnati
State
OH
Country
United States
Zip Code
45221
Kang, Wenjing; Pei, Xing; Rusinek, Cory A et al. (2017) Determination of Lead with a Copper-Based Electrochemical Sensor. Anal Chem 89:3345-3352
Kang, Wenjing; Rusinek, Cory; Bange, Adam et al. (2017) Determination of manganese by cathodic stripping voltammetry on a microfabricated platinum thin-film electrode. Electroanalysis 29:686-695
Pei, Xing; Kang, Wenjing; Yue, Wei et al. (2014) Disposable copper-based electrochemical sensor for anodic stripping voltammetry. Anal Chem 86:4893-900
Kang, Wenjing; Pei, Xing; Bange, Adam et al. (2014) Copper-based electrochemical sensor with palladium electrode for cathodic stripping voltammetry of manganese. Anal Chem 86:12070-7
Pei, Xing; Kang, Wenjing; Yue, Wei et al. (2014) Improving Reproducibility of Lab-on-a-Chip Sensor with Bismuth Working Electrode for Determining Zn in Serum by Anodic Stripping Voltammetry. J Electrochem Soc 161:B3160-B3166
Jothimuthu, Preetha; Wilson, Robert A; Herren, Josi et al. (2013) Zinc Detection in Serum by Anodic Stripping Voltammetry on Microfabricated Bismuth Electrodes. Electroanalysis 25:
Zerhusen, Benjamin; de Silva, Geethanga; Pei, Xing et al. (2013) Point-of-Care device for quantification of zinc in serum. Conf Proc IEEE Eng Med Biol Soc 2013:4171-4
Kang, Wenjing; Pei, Xing; Yue, Wei et al. (2013) Lab-on-a-Chip Sensor with Evaporated Bismuth Film Electrode for Anodic Stripping Voltammetry of Zinc. Electroanalysis 25:2586-2594
Yue, Wei; Bange, Adam; Riehl, Bill L et al. (2013) The Application of Nafion Metal Catalyst Free Carbon Nanotube Modified Gold Electrode: Voltammetric Zinc Detection in Serum. Electroanalysis 25:2259-2267
Kadambi, Pooja; Lovelace, Joseph A; Beyette, Fred R (2013) Changes in behavior of evoked potentials in the brain as a possible indicator of fatigue in people. Conf Proc IEEE Eng Med Biol Soc 2013:6381-4

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