Abstract

The regulation of lymphocyte migration by macrophages will be studied in vitro using the random migration assay and the modified Boyden chemotaxis assay. The culture supernatants of peritoneal macrophages contain a factor(s) that is(are) chemokinetic and chemotactic for lymphocytes as observed in our laboratory. The chemokinetic activity is also present in the culture supernatants of J744A and P388D1 macrophage-like cell lines. In the proposed project the macrophage factor(s)1 will be isolated, purified and characterized, and its effect on the migration of different lymphocyte populations will be studied using lymphocyte subpopulations isolated by conventional methods and using cloned T cells. The in vivo effect of the purified factor(s) on the migration and distribution of lymphocytes will also be studied. The mechanisms of action of the factor(s) on the modulation of lymphocyte migration will be examined by studying the changes in the contractile and cytoskeletal structure of the affected cells. The proposed study is intended to gain new information on the mechanism and regulation of lymphocyte migration and localization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Unknown (R23)
Project #
5R23AI020343-02
Application #
3445489
Study Section
Experimental Immunology Study Section (EI)
Project Start
1984-04-01
Project End
1987-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
2
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
Illinois State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Normal
State
IL
Country
United States
Zip Code
61790

  Publications

Davis, J M; St John, J; Cheung, H T (1990) Haptotactic activity of fibronectin on lymphocyte migration in vitro. Cell Immunol 129:67-79
Ye, S; Cheung, H T (1989) Regulation of lymphocyte motility by macrophages: characterization of a lymphocyte migration inhibitory factor derived from a macrophage-like cell line. Cell Immunol 122:231-43
Liao, N S; ST John, J; McCarthy, J B et al. (1989) Adhesion of lymphoid cells to the carboxyl-terminal heparin-binding domains of fibronectin. Exp Cell Res 181:348-61
Ye, S; Applegren, R R; Davis, J M et al. (1989) Modulation of lymphocyte motility by beta-endorphin and met-enkephalin. Immunopharmacology 17:81-9
Pahlavani, M A; Cheung, T H; Chesky, J A et al. (1988) Influence of exercise on the immune function of rats of various ages. J Appl Physiol 64:1997-2001
Li, D D; Chien, Y K; Gu, M Z et al. (1988) The age-related decline in interleukin-3 expression in mice. Life Sci 43:1215-22
Cheung, H T; Twu, J S (1987) Modulation of lymphocyte motility by macrophages. Cell Immunol 109:295-305
Cheung, H T; Rehwaldt, C A; Twu, J S et al. (1987) Aging and lymphocyte cytoskeleton: age-related decline in the state of actin polymerization in T lymphocytes from Fischer F344 rats. J Immunol 138:32-6
Pahlavani, M A; Richardson, A; Cheung, H T (1987) Age-dependent changes of the mesenteric lymph node of Fischer F344 rats: morphological and histometric analysis. Mech Ageing Dev 39:137-46
Liao, N S; St John, J; Du, Z J et al. (1987) Adhesion of lymphoid cell lines to fibronectin-coated substratum: biochemical and physiological characterization and the identification of a 140-kDa fibronectin receptor. Exp Cell Res 171:306-20

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