The major objective of ths proposal is to enhance and expand the capabilities of an institutional Microscopy Shared Resource Facility (SRF) at Mount Sinai School of Medicine. Improvements to this core facility will significantly enhance the research efforts of NCI-funded (and other NIH-funded) investigators throughout the institution. Improvements will be developed on three fronts: a) live cell imaging; b) molecular localization, and c) digital image acquisition and image analysis. In addition to enhancing the capabilities of the facility, overall operating costs will be lowered, thus making the facility more accessible (financially) to junior investigators.
The specific aims are: 1) to provide increased access by NCI- and other NIH-funded investigators to state-of-the-art instrumentation and high quality, cost effective microscopy services by reducing user fees charged; 2) to educate and train NCI-funded investigators in the use of advanced microscopy and imaging techniques in cancer research; 3) to promote NCI-funded use of the Microscopy SRF by adding an NCI-funded investigator to the Microscopy SRF advisory committee; 4) to enhance live-cell (live-tissue) imaging capabilities, including multi-photon laser scanning microscopy, by providing equipment to support vital imaging (e.g. cell culture equipment, heated microscope stages) on site and by hiring a technician to assist with live cell / confocal / multi-photon imaging; 5) to improve user-friendliness of electron microscopy and decrease turn-around time for EM results by installing digital image acquisition system (cCCD camera) on the electron microscope; and 6) to enhance ultrastructural immunolocalization capabilities by including cyro-fixation, freeze-substitution, and cryo-ultramicrotomy techniques to our options for immunolocalization by electron microscopy. Representative studies from 16 NCI funded labs (representing 7 departments at MSSM) have been highlighted in the Research Plan of this grant proposal. Successful organizational, operational, and business plans are already in place. In addition to the use training activities already well established within the core, plans are being developed to expand the educational efforts of the core (including a graduate school course, lectures, and multi-media learning materials).
|Horng, Sam; Therattil, Anthony; Moyon, Sarah et al. (2017) Astrocytic tight junctions control inflammatory CNS lesion pathogenesis. J Clin Invest 127:3136-3151|
|Laitman, Benjamin M; Asp, Linnéa; Mariani, John N et al. (2016) The Transcriptional Activator Krüppel-like Factor-6 Is Required for CNS Myelination. PLoS Biol 14:e1002467|
|Jahanshahi, Maryam; Hsiao, Kuangfu; Jenny, Andreas et al. (2016) The Hippo Pathway Targets Rae1 to Regulate Mitosis and Organ Size and to Feed Back to Regulate Upstream Components Merlin, Hippo, and Warts. PLoS Genet 12:e1006198|
|Varghese, Merina; Santa-Maria, Ismael; Ho, Lap et al. (2016) Extracellular Tau Paired Helical Filaments Differentially Affect Tau Pathogenic Mechanisms in Mitotic and Post-Mitotic Cells: Implications for Mechanisms of Tau Propagation in the Brain. J Alzheimers Dis 54:477-96|
|Lobatto, Mark E; Calcagno, Claudia; Millon, Antoine et al. (2015) Atherosclerotic plaque targeting mechanism of long-circulating nanoparticles established by multimodal imaging. ACS Nano 9:1837-47|
|Sanchez-Gaytan, Brenda L; Fay, Francois; Lobatto, Mark E et al. (2015) HDL-mimetic PLGA nanoparticle to target atherosclerosis plaque macrophages. Bioconjug Chem 26:443-51|
|Chapouly, Candice; Tadesse Argaw, Azeb; Horng, Sam et al. (2015) Astrocytic TYMP and VEGFA drive blood-brain barrier opening in inflammatory central nervous system lesions. Brain 138:1548-67|
|Duivenvoorden, Raphaël; Tang, Jun; Cormode, David P et al. (2014) A statin-loaded reconstituted high-density lipoprotein nanoparticle inhibits atherosclerotic plaque inflammation. Nat Commun 5:3065|
|Nair-Gupta, Priyanka; Baccarini, Alessia; Tung, Navpreet et al. (2014) TLR signals induce phagosomal MHC-I delivery from the endosomal recycling compartment to allow cross-presentation. Cell 158:506-21|
|Papa, Luena; Hahn, Mary; Marsh, Ellen L et al. (2014) SOD2 to SOD1 switch in breast cancer. J Biol Chem 289:5412-6|
Showing the most recent 10 out of 233 publications