The Primate Embryo Gene Expression Resource (PREGER) advances the biology of nonhuman primate (NHP) and human oocytes and embryos, recognizing the significant limitations of rodent and other models to inform us about key aspects of human reproductive biology. PREGER overcomes the severe cost limitations of nonhuman primate oocyte and embryo biology and the legal and ethical restrictions associated with human studies, and have dramatically increased our understanding of nonhuman primate &human oocytes and embryos. PREGER provides to a growing set of users worldwide (1) an extensive set of >200 cDNA libraries, plus molecular reagents, methodologies, databases, and other resources to advance research in NHP embryology. (2) a large and expanding gene expression database base related to NHP embryogenesis and molecular determinants of oocyte and embryo quality, (3) specialized molecular services to the reproductive biology community, and (4) specialized training to expand the number of investigators in the field, 25% of whom have been clinically oriented scientists. In this proposal we request support to continue providing libraries and other reagents, as well as services to those scientists who require access to these specialized collections of materials and methods, which are applicable to studying primate and mammalian oocytes and embryos. We will also expand the database resource to include a new reproductive toxicology database, which will facilitate studies of endocrine disrupting and obesogen compounds of great interest to the National Toxicology Program for potential roles in developmental origins of human disease. We will also develop a new biofunction pathways database linking genes to cellular processes, and develop a crucially important new protein expression database to accompany mRNA expression data. Links will also be made to other databases related to human disease. Last, we will continue a summer course and expand the human resource development component by producing online instructional videos. Through these activities PREGER will continue to provide a highly productive, unique, and innovative resource with strong translational relevance.
Molecular studies of gene expression in primate oocytes and embryos provide unique information for addressing human reproductive health &fertility. It is also useful for evaluating risks related to environmental factors and developmental origins of adult human disease. PREGER provides essential reagents, services, databases, and training to advance human reproductive health research.
|Ruebel, Meghan L; Schall, Peter Z; Midic, Uros et al. (2018) Transcriptome analysis of rhesus monkey failed-to-mature oocytes: deficiencies in transcriptional regulation and cytoplasmic maturation of the oocyte mRNA population. Mol Hum Reprod 24:478-494|
|Midic, Uros; Goheen, Benjamin; Vincent, Kailey A et al. (2018) Changes in gene expression following long-term in vitro exposure of Macaca mulatta trophoblast stem cells to biologically relevant levels of endocrine disruptors. Reprod Toxicol 77:154-165|
|Midic, Uros; VandeVoort, Catherine A; Latham, Keith E (2018) Determination of single embryo sex in Macaca mulatta and Mus musculus RNA-Seq transcriptome profiles. Physiol Genomics 50:628-635|
|Ding, Deqiang; Liu, Jiali; Midic, Uros et al. (2018) TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. Nat Commun 9:127|
|Midic, Uros; Hung, Pei-Hsuan; Vincent, Kailey A et al. (2017) Quantitative assessment of timing, efficiency, specificity and genetic mosaicism of CRISPR/Cas9-mediated gene editing of hemoglobin beta gene in rhesus monkey embryos. Hum Mol Genet 26:2678-2689|
|Ding, Deqiang; Liu, Jiali; Dong, Kunzhe et al. (2017) PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun 8:819|
|Latham, Keith E (2016) Stress signaling in mammalian oocytes and embryos: a basis for intervention and improvement of outcomes. Cell Tissue Res 363:159-67|
|Midic, Uros; Vincent, Kailey A; VandeVoort, Catherine A et al. (2016) Effects of long-term endocrine disrupting compound exposure on Macaca mulatta embryonic stem cells. Reprod Toxicol 65:382-393|
|VandeVoort, Catherine A; Grimsrud, Kristin N; Midic, Uros et al. (2015) Transgenerational effects of binge drinking in a primate model: implications for human health. Fertil Steril 103:560-9|
|VandeVoort, Catherine A; Mtango, Namdori R; Midic, Uros et al. (2015) Disruptions in follicle cell functions in the ovaries of rhesus monkeys during summer. Physiol Genomics 47:102-12|
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