The cryo-EM determination of atomic structures by single-particle methods represents a revolution in structural biology. The method relies on the astonishing ability of numerical computations to yield high- resolution density maps from collections of extremely noisy ?particle? images. This ability however carries with it a real danger of spurious results emerging from the black box of image-processing and reconstruction software. We propose to develop an educational curriculum including video, software and an e-book that provides a rigorous treatment of the mathematical foundations of cryo-EM image processing and reconstruction. The goal of our instructional efforts is to ground students and researchers in the understanding of the principles and best-practice methods in the computational processing that leads to molecular structures.

Public Health Relevance

The cryo-EM determination of atomic structures by single-particle methods represents a revolution in structural biology. The method relies on the astonishing ability of numerical computations to yield high- resolution density maps from collections of extremely noisy ?particle? images. We propose to develop a curriculum that will ground students and researchers in the understanding of fundamental principles and the best-practice methods in the computational processing of cryo-EM data.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Education Projects (R25)
Project #
5R25EY029125-02
Application #
9688544
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Araj, Houmam H
Project Start
2018-05-01
Project End
2021-04-30
Budget Start
2019-05-01
Budget End
2020-04-30
Support Year
2
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Yale University
Department
Physiology
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520