Abstract

This proposal is to investigate the roles of the JNK cascade in TNF-alpha-induced apoptosis in vitro cell culture systems. This research is based on a publication report that TNF-alpha-induced apoptosis can be blocked by the dominant negative mutants of c-Jun and JNKK.
The specific aims of this proposal are: 1) to investigate the effect of FADD on the JNK cascade in TNF-alpha-induced apoptosis, 2) to investigate the effect of selective inhibition of the JNK cascade on TNF-alpha-induced apoptosis, and 3) to investigate the effect of selective activation of the JNK cascade on TNF-alpha-induced apoptosis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
7R29CA073740-04
Application #
6172900
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Program Officer
Spalholz, Barbara A
Project Start
1997-06-01
Project End
2002-05-31
Budget Start
1999-11-12
Budget End
2000-05-31
Support Year
4
Fiscal Year
2000
Total Cost
$52,932
Indirect Cost

  Institution

Name
University of Chicago
Department
Miscellaneous
Type
Schools of Medicine
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Li, Jing; Johnson, Xa Dwight; Iazvovskaia, Svetlana et al. (2003) Signaling intermediates required for NF-kappa B activation and IL-8 expression in CF bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol 284:L307-15
Page, Kristen; Li, Jing; Zhou, Limei et al. (2003) Regulation of airway epithelial cell NF-kappa B-dependent gene expression by protein kinase C delta. J Immunol 170:5681-9
Tang, Fangming; Tang, Guilin; Xiang, Jialing et al. (2002) The absence of NF-kappaB-mediated inhibition of c-Jun N-terminal kinase activation contributes to tumor necrosis factor alpha-induced apoptosis. Mol Cell Biol 22:8571-9
Purcell, N H; Tang, G; Yu, C et al. (2001) Activation of NF-kappa B is required for hypertrophic growth of primary rat neonatal ventricular cardiomyocytes. Proc Natl Acad Sci U S A 98:6668-73
Purcell, N H; Yu, C; He, D et al. (2001) Activation of NF-kappaB by hepatitis B virus X protein through an IkappaB kinase-independent mechanism. Am J Physiol Gastrointest Liver Physiol 280:G669-77
Tang, G; Yang, J; Minemoto, Y et al. (2001) Blocking caspase-3-mediated proteolysis of IKKbeta suppresses TNF-alpha-induced apoptosis. Mol Cell 8:1005-16
Kim, H L; Vander Griend, D J; Yang, X et al. (2001) Mitogen-activated protein kinase kinase 4 metastasis suppressor gene expression is inversely related to histological pattern in advancing human prostatic cancers. Cancer Res 61:2833-7
Zheng, C; Xiang, J; Hunter, T et al. (1999) The JNKK2-JNK1 fusion protein acts as a constitutively active c-Jun kinase that stimulates c-Jun transcription activity. J Biol Chem 274:28966-71
Nemoto, S; Sheng, Z; Lin, A (1998) Opposing effects of Jun kinase and p38 mitogen-activated protein kinases on cardiomyocyte hypertrophy. Mol Cell Biol 18:3518-26
Nemoto, S; DiDonato, J A; Lin, A (1998) Coordinate regulation of IkappaB kinases by mitogen-activated protein kinase kinase kinase 1 and NF-kappaB-inducing kinase. Mol Cell Biol 18:7336-43

Showing the most recent 10 out of 12 publications