The aim of this research project is to elucidate the mechanism of carbon mon-oxide-mediated brain lipid peroxidation, which we have identified in a rat model of CO poisoning. CO is the leading cause of death by poisoning in the United States, and 12% or more of survivors suffer delay neurologic deterioration after the acute insult. The mechanism for this delayed process is unknown, but it is hypothesized to be an oxidative injury. Our hypothesis is that lipid peroxidation in our model represents what is popularly described as an """"""""ischemia-reperfusion"""""""" phenomenon. We propose to study the time course for lipid peroxidation in greater detail over the days following CO exposure, and to correlate lipid peroxidation with functional changes using (14C) 2-deoxyglucose imaging and phosphorous NMR spectroscopy. The mechanism for brain lipid peroxidation will be studied using various techniques. We will evaluate the role of xanthine oxidase by assessing the impact of enzyme inhibition, using tungsten, on the development of lipid peroxidation after CO poisoning. The time course of xanthin dehydrogenase to xanthine oxidase conversion after CO poisoning will also be measured. We will evaluate the involvement of oxidants by measuring brain H202 at various times subsequent to CO exposure; and also by determining the impact of infusion of dimethylthiourea, an H202 scavenger, desferrioxamine, an iron chelator, and liposome-encapsulated antioxidant enzymes (superoxide dismutase and/or catalase) on CO-mediated lipid peroxidation. We also propose to study in greater detail our observation involving hyperbaric oxygen (HBO) antagonism of CO-mediated lipid peroxidation. These studies will involve further in vivo experiments, administration of HBO at different times after CO poisoning; and further in vitro experiments using xanthine oxidase generated radicals to assess the impact of 02 concentration and PH on linoleic acid peroxidation.

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29ES005211-05
Application #
3465309
Study Section
Toxicology Subcommittee 2 (TOX)
Project Start
1989-07-01
Project End
1994-06-30
Budget Start
1993-07-01
Budget End
1994-06-30
Support Year
5
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Mendelman, A; Zarchin, N; Rifkind, J et al. (2000) Brain multiparametric responses to carbon monoxide exposure in the aging rat. Brain Res 867:217-22
Meilin, S; Rogatsky, G G; Thom, S R et al. (1996) Effects of carbon monoxide on the brain may be mediated by nitric oxide. J Appl Physiol 81:1078-83
Mayevsky, A; Meilin, S; Rogatsky, G G et al. (1995) Multiparametric monitoring of the awake brain exposed to carbon monoxide. J Appl Physiol 78:1188-96
Hardy, K R; Thom, S R (1994) Pathophysiology and treatment of carbon monoxide poisoning. J Toxicol Clin Toxicol 32:613-29
Ischiropoulos, H; Mendiguren, I; Fisher, D et al. (1994) Role of neutrophils and nitric oxide in lung alveolar injury from smoke inhalation. Am J Respir Crit Care Med 150:337-41
Thom, S R; Mendiguren, I; Van Winkle, T et al. (1994) Smoke inhalation with a concurrent systemic stress results in lung alveolar injury. Am J Respir Crit Care Med 149:220-6
Thom, S R (1993) Leukocytes in carbon monoxide-mediated brain oxidative injury. Toxicol Appl Pharmacol 123:234-47
Thom, S R (1993) Functional inhibition of leukocyte B2 integrins by hyperbaric oxygen in carbon monoxide-mediated brain injury in rats. Toxicol Appl Pharmacol 123:248-56
Thom, S R (1992) Inert gas enhancement of superoxide radical production. Arch Biochem Biophys 295:391-6
Thom, S R (1992) Dehydrogenase conversion to oxidase and lipid peroxidation in brain after carbon monoxide poisoning. J Appl Physiol 73:1584-9