Paramagnetic Ions Use in NMR Structure Determination

Gochin, Miriam

Abstract

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of General Medical Sciences (NIGMS)
• Type: First Independent Research Support & Transition (FIRST) Awards (R29)
• Project #: 5R29GM053164-02
• Application #: 2192458
• Study Section: Molecular and Cellular Biophysics Study Section (BBCA)
• Project Start: 1995-07-01
• Project End: 2000-06-30
• Budget Start: 1996-07-01
• Budget End: 1997-06-30
• Support Year: 2
• Fiscal Year: 1996

Institution

• Name: University of the Pacific-San Francisco
• Department: Microbiology/Immun/Virology
• Type: Schools of Dentistry
• City: San Francisco
• State: CA
• Country: United States
• Zip Code: 94115

Publications

Gochin, Miriam; Khorosheva, Valentina; Case, Martin A (2002) Structural characterization of a paramagnetic metal-ion-assembled three-stranded alpha-helical coiled coil. J Am Chem Soc 124:11018-28

Gochin, M (2000) A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic resonance. Structure 8:441-52

Tu, K; Gochin, M (1999) Structure determination by restrained molecular dynamics using NMR pseudocontact shifts as experimentally determined constraints. J Am Chem Soc 121:9276-85

Buchko, G W; Daughdrill, G W; de Lorimier, R et al. (1999) Interactions of human nucleotide excision repair protein XPA with DNA and RPA70 Delta C327: chemical shift mapping and 15N NMR relaxation studies. Biochemistry 38:15116-28

Gochin, M (1998) Nuclear magnetic resonance characterization of a paramagnetic DNA-drug complex with high spin cobalt;assignment of the 1H and 31P NMR spectra, and determination of electronic, spectroscopic and molecular properties. J Biomol NMR 12:243-57