Viral infections such as influenza are characterized by an increased bronchoconstriction to a variety of stimuli in vivo. Although this hyperreactivity is usually temporary, viral infections in childhood are associated with development of asthma. The hyperreactivity to any specific agonist could result from direct effects on smooth muscle or effects on cells that control smooth muscle contraction. A major indirect route of hyperreactivity Is via the vagus nerve. The vagus nerve releases acetylcholine that binds to muscarinic receptors on the smooth muscle resulting in bronchoconstriction. In preliminary data I have shown that the amount of acetylcholine released by the nerve terminals is regulated by muscarinic receptors which are located on the nerve terminals, and that these neuronal receptors are a different subtype than the muscarinic receptors on the smooth muscle. The neuronal muscarinic receptors are inhibitory in that when stimulated, they limit the amount of acetylcholine released from the nerves. Damage to these neuronal receptors would remove both the tonic inhibition and the negative feedback that occurs with all stimuli which increase vagal activity. It is my hypothesis that the pathologic absence of this normal negative feedback will result in airways hyperreactivity. Viral infections such as influenza and parainfluenza, temporarily increases the bronchoconstriction produced by a variety of stimuli. The mechanism of viral-induced airway hyperreactivity is poorly understood. However, there is evidence to suggest that part of the defect lies in the vagus nerves. Viruses contain enzymes on their surface which can damage cells and receptors on cells. One of these enzymes, neuraminidase, can damage receptors like the muscarinic receptor which inhibits acetylcholine release from the vagus nerves in the lung. Neuraminidase may be produced in large quantities during viral infection as new viruses grow in the airways. I have shown that during viral infection neuronal inhibitory muscarinic receptors are not functioning. The experiments in this proposal are designed to test whether damage to these receptors on the vagus nerves play an important role in the reactivity of the lungs, whether this effect is due specifically to inhibition of neuronal muscarinic receptors by neuraminidase, and what role neuraminidase plays in other models of airways disease, especially antigen challenge. This grant proposes studying muscarinic receptor function in vivo and in vitro as well as studying the receptors at a genetic level. It is expected that results from this study will provide important new insights into the mechanism of airway hyperreactivity induced by viral infection and may yield results which further our understanding of other models of airway disease.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
First Independent Research Support & Transition (FIRST) Awards (R29)
Project #
5R29HL044727-03
Application #
3473102
Study Section
Pathology A Study Section (PTHA)
Project Start
1991-08-01
Project End
1996-07-31
Budget Start
1993-08-01
Budget End
1994-07-31
Support Year
3
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Johns Hopkins University
Department
Type
Schools of Public Health
DUNS #
045911138
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Adamko, D J; Yost, B L; Gleich, G J et al. (1999) Ovalbumin sensitization changes the inflammatory response to subsequent parainfluenza infection. Eosinophils mediate airway hyperresponsiveness, m(2) muscarinic receptor dysfunction, and antiviral effects. J Exp Med 190:1465-78
Costello, R W; Evans, C M; Yost, B L et al. (1999) Antigen-induced hyperreactivity to histamine: role of the vagus nerves and eosinophils. Am J Physiol 276:L709-14
Jacoby, D B; Xiao, H Q; Lee, N H et al. (1998) Virus- and interferon-induced loss of inhibitory M2 muscarinic receptor function and gene expression in cultured airway parasympathetic neurons. J Clin Invest 102:242-8
Costello, R W; Fryer, A D; Belmonte, K E et al. (1998) Effects of tachykinin NK1 receptor antagonists on vagal hyperreactivity and neuronal M2 muscarinic receptor function in antigen challenged guinea-pigs. Br J Pharmacol 124:267-76
Costello, R W; Schofield, B H; Kephart, G M et al. (1997) Localization of eosinophils to airway nerves and effect on neuronal M2 muscarinic receptor function. Am J Physiol 273:L93-103
Belmonte, K E; Jacoby, D B; Fryer, A D (1997) Increased function of inhibitory neuronal M2 muscarinic receptors in diabetic rat lungs. Br J Pharmacol 121:1287-94
Fryer, A D; Costello, R W; Yost, B L et al. (1997) Antibody to VLA-4, but not to L-selectin, protects neuronal M2 muscarinic receptors in antigen-challenged guinea pig airways. J Clin Invest 99:2036-44
Evans, C M; Fryer, A D; Jacoby, D B et al. (1997) Pretreatment with antibody to eosinophil major basic protein prevents hyperresponsiveness by protecting neuronal M2 muscarinic receptors in antigen-challenged guinea pigs. J Clin Invest 100:2254-62
Okanlami, O A; Fryer, A D; Hirshman, C (1996) Interaction of nondepolarizing muscle relaxants with M2 and M3 muscarinic receptors in guinea pig lung and heart. Anesthesiology 84:155-61
Kahn, R M; Okanlami, O A; Jacoby, D B et al. (1996) Viral infection induces dependence of neuronal M2 muscarinic receptors on cyclooxygenase in guinea pig lung. J Clin Invest 98:299-307

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