We are highly motivated to develop innovative research tools to meet the needs of solving challenging biological and biomedical problems, as we have done in the past and will be doing in the future. Currently, by developing and employing high-speed super-resolution microscopy techniques, our research aims to solve two critical transport mechanisms involving three sub- cellular organelles in eukaryotic cells: nucleus, cytoplasm and primary cilium. Macromolecular trafficking among these compartments is suggested to be gated by two unique machineries. One is the nuclear pore complex (NPC) embedded in the nuclear envelope that mediates the bidirectional trafficking of proteins and RNAs between the cytoplasm and the nucleus; the other is the transition zone (TZ) located at the base of cilium that regulates the entry of membrane and cytosolic proteins into the cilium. Due to the challenges in elucidating kinetics and real-time transport routes for macromolecules through the sub-micrometer NPC or TZ in live cells, however, the fundamental gating mechanisms in either of these two machineries, remain obscure. Moreover, these transport mechanisms are not only the fundamental unanswered questions in cell biology, but also are closely associated with human diseases. For example, dysfunction of the nuclear transport through the NPC are linked to numerous human diseases including leukemias, cancers, and primary biliary cirrhosis. Also, defects in ciliary structure and/or function causes a variety of diseases (called ciliopathies) such as cystic kidney disease, nephronophthisis (NPHP), and retinitis pigmentosa. Thus, the fundamental knowledge of understanding the gating mechanisms in these transport systems is urgently needed to further develop therapeutics for the human diseases. In this project, we will employ and further develop high-speed super-resolution fluorescence microscopy techniques to unravel these fundamental transport mechanisms.
In our laboratory, we are highly motivated to develop innovative research tools to meet the needs of solving challenging biological and biomedical problems, as we have done in the past and will be doing in the future. Currently, by developing and employing high-speed super- resolution microscopy techniques, our research aims to solve two critical transport mechanisms among three sub-cellular organelles in eukaryotic cells: nucleocytoplasmic transport mechanism between cytoplasm and nucleus and gating mechanism between cytoplasm and primary cilium.
|Ruba, Andrew; Luo, Wangxi; Yang, Weidong (2018) Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium. J Vis Exp :|
|Mudumbi, Krishna C; Yang, Weidong (2017) Determination of Membrane Protein Distribution on the Nuclear Envelope by Single-Point Single-Molecule FRAP. Curr Protoc Cell Biol 76:21.11.1-21.11.13|
|Ma, Jiong; Kelich, Joseph M; Junod, Samuel L et al. (2017) Super-resolution mapping of scaffold nucleoporins in the nuclear pore complex. J Cell Sci 130:1299-1306|
|Luo, Wangxi; Ruba, Andrew; Takao, Daisuke et al. (2017) Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium. Sci Rep 7:15793|