Abstract

Investigator's Specific Aims): The overall goals of this project are to use genetic strategies to study the role of leukocyte and endothelial cell adhesion molecules in normal biology and in disease processes.
Five Specific Aims are proposed: (1) Develop mutations in all three mouse selectin genes. (2) Perform phenotypic evaluation of knockout mice, including routine pathology, leukocyte counts, peritonitis, pneumonia, delayed type hypersensitivity reactions, and intravital microscopy. (3) Investigate the pathogenesis of psoriasis-like skin disease in CD18 deficient mice, specifically searching for a modifier gene or genes using a genomic-wide linkage strategy. (4) Develop cell-specific and inducible mutations using the loxP/Cre recombinase system, including an endothelial cell-specific mutation for VCAM-1 and an inducible mutations for ICAM-1. (5) Evaluate the role of adhesion molecules in the pathogenesis of atherosclerosis using apolipoprotein E-deficient mice.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37AI032177-10
Application #
6341616
Study Section
Special Emphasis Panel (ZRG2-BIOL-1 (01))
Program Officer
Ridge, John P
Project Start
1991-07-01
Project End
2001-12-31
Budget Start
2001-01-01
Budget End
2001-12-31
Support Year
10
Fiscal Year
2001
Total Cost
$337,859
Indirect Cost

  Institution

Name
Baylor College of Medicine
Department
Genetics
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Dickinson, Mary E; Flenniken, Ann M; Ji, Xiao et al. (2017) Corrigendum: High-throughput discovery of novel developmental phenotypes. Nature 551:398
Yang, Tao; Mendoza-Londono, Roberto; Lu, Huifang et al. (2010) E-selectin ligand-1 regulates growth plate homeostasis in mice by inhibiting the intracellular processing and secretion of mature TGF-beta. J Clin Invest 120:2474-85
Garcia-Palacios, Veronica; Chung, Ho Yeon; Choi, Sun Jin et al. (2007) Eosinophil chemotactic factor-L (ECF-L) enhances osteoclast formation by increasing in osteoclast precursors expression of LFA-1 and ICAM-1. Bone 40:316-22
Palacios, Veronica Garcia; Chung, Ho Yeon; Choi, Sun Jin et al. (2006) Eosinophil chemotactic factor-L (ECF-L) enhances osteoclast formation by increasing ICAM-1 expression. Ann N Y Acad Sci 1068:240-3
Robker, Rebecca L; Collins, Robert G; Beaudet, Arthur L et al. (2004) Leukocyte migration in adipose tissue of mice null for ICAM-1 and Mac-1 adhesion receptors. Obes Res 12:936-40
Dunne, Jessica L; Collins, Robert G; Beaudet, Arthur L et al. (2003) Mac-1, but not LFA-1, uses intercellular adhesion molecule-1 to mediate slow leukocyte rolling in TNF-alpha-induced inflammation. J Immunol 171:6105-11
Dunne, Jessica L; Ballantyne, Christie M; Beaudet, Arthur L et al. (2002) Control of leukocyte rolling velocity in TNF-alpha-induced inflammation by LFA-1 and Mac-1. Blood 99:336-41
Davis, R J; Shen, W; Sandler, Y I et al. (2001) Dach1 mutant mice bear no gross abnormalities in eye, limb, and brain development and exhibit postnatal lethality. Mol Cell Biol 21:1484-90
Collins, R G; Jung, U; Ramirez, M et al. (2001) Dermal and pulmonary inflammatory disease in E-selectin and P-selectin double-null mice is reduced in triple-selectin-null mice. Blood 98:727-35
Papayannopoulou, T; Priestley, G V; Nakamoto, B et al. (2001) Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood 97:1282-8

Showing the most recent 10 out of 34 publications