Detailed information is available regarding the mechanisms of regulating specific genes in bacteria. Much less is known about how these individual control systems interact so as to coordinate the functions of multigene systems. We propose to genetically approach control of several multigene systems and how they might interact. We will work on 1) the histidine operon and how it interacts with the related purine pathway and the cells control of cell division, 2) the purine pathway per se will be investigated to determine how the many genes in its separate branches are controlled, 3) a new system of gene regulation will be investigated which we believe is related to purine metabolism and involves structural alteration of DNA. The system seems to cause reversible inactivation of cryptic prophages, 4) the NAD pathway involves not only biosynthesis but also recycling pathway for salvage of pyridine nucleotides. This system is involved in DNA repair as well as oxidation/reduction reactions. The control of this complex pathway and its integration with cellular regulatory mechanisms will be pursued. All of these endeavors will involve a heavily genetic approach employing transposable elements, some of which form operon fusions of the lac operon to the promoter of the target gene. Many of the methods used are one developed in this lab.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM023408-18
Application #
2174085
Study Section
Special Emphasis Panel (NSS)
Project Start
1977-01-01
Project End
1994-12-31
Budget Start
1994-01-01
Budget End
1994-12-31
Support Year
18
Fiscal Year
1994
Total Cost
Indirect Cost
Name
University of Utah
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Li, Chan; Edwards, Michelle D; Jeong, Hotcherl et al. (2007) Identification of mutations that alter the gating of the Escherichia coli mechanosensitive channel protein, MscK. Mol Microbiol 64:560-74
Grose, Julianne H; Joss, Lisa; Velick, Sidney F et al. (2006) Evidence that feedback inhibition of NAD kinase controls responses to oxidative stress. Proc Natl Acad Sci U S A 103:7601-6
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Grose, Julianne H; Bergthorsson, Ulfar; Xu, Yaping et al. (2005) Assimilation of nicotinamide mononucleotide requires periplasmic AphA phosphatase in Salmonella enterica. J Bacteriol 187:4521-30
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Park, U E; Roth, J R; Olivera, B M (1988) Salmonella typhimurium mutants lacking NAD pyrophosphatase. J Bacteriol 170:3725-30

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