In this project, understanding of the structure/function relationships for the major RNA's in translation is sought. The method to be used is site-directed mutagenesis of tRNA, mRNA, or rRNA genes, selection or screening of function, then quantitative comparison of action of altered RNA products in vivo. There are three major divisions to the work. 1. The extended anticodon hypothesis. In the first, tRNA genes will be manipulated to show that a second class of anticodons require an optimal anticodon arm structure for translational efficiency, as do Uxx codons. The Uxx series has been investigated in the preceding grant period. Ribosomal RNA:tRNA interaction, suggested by these prior results, will be tested by genetic means. 2. The A24 mutation. In the second, an important tRNA mutant which seems to be an exception to the generalization of the first section, will be investigated to see if the hypothesis needs to be generalized to embrace it. 3. Codon choice and codon context. In the third section of the grant, both messages and tRNA's will be altered to investigate the differential effects of codon choice and codon context on gene expression. Message: tRNA interactions and tRNA:tRNA interactions will be investigated, and a new theory will be tested to connect the local rate of translation to gene expression.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37GM030881-07
Application #
3484704
Study Section
Physiological Chemistry Study Section (PC)
Project Start
1982-07-01
Project End
1992-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
7
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of Colorado at Boulder
Department
Type
Schools of Arts and Sciences
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309
Smit, Sandra; Yarus, Michael; Knight, Rob (2006) Natural selection is not required to explain universal compositional patterns in rRNA secondary structure categories. RNA 12:1-14
Janas, Tadeusz; Janas, Teresa; Yarus, Michael (2006) Specific RNA binding to ordered phospholipid bilayers. Nucleic Acids Res 34:2128-36
Knight, Rob; De Sterck, Hans; Markel, Rob et al. (2005) Abundance of correctly folded RNA motifs in sequence space, calculated on computational grids. Nucleic Acids Res 33:5924-35
Janas, Teresa; Janas, Tadeusz; Yarus, Michael (2004) A membrane transporter for tryptophan composed of RNA. RNA 10:1541-9
Janas, Tadeusz; Yarus, Michael (2003) Visualization of membrane RNAs. RNA 9:1353-61
Jadhav, Vasant R; Yarus, Michael (2002) Coenzymes as coribozymes. Biochimie 84:877-88
Illangasekare, Mali; Yarus, Michael (2002) Phenylalanine-binding RNAs and genetic code evolution. J Mol Evol 54:298-311
Jadhav, Vasant R; Yarus, Michael (2002) Acyl-CoAs from coenzyme ribozymes. Biochemistry 41:723-9
Kumar, R K; Yarus, M (2001) RNA-catalyzed amino acid activation. Biochemistry 40:6998-7004
Yarus, M (2001) On translation by RNAs alone. Cold Spring Harb Symp Quant Biol 66:207-15

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