Abstract

This renewal application seeks to investigate how vitamin A regulates mucous cell differentiation in airway epithelium. Two stages of mucous cell differentiation are proposed. In the first stage, cells are unable to differentiate into mucous cells in response to vitamin A. However, they can be transformed into mucin-producing cells by microinjection of RNA from differentiated mucous cells. This first stage is therefore termed the """"""""competent"""""""" stage of mucous cell differentiation, and vitamin A can cause immortalized airway epithelial cell lines to become competent. In the second """"""""progressive"""""""" stage, vitamin A induces mucous cell differentiation. Dr. Wu has identified one to three genes from progressive stage cells which cause stage 1 cells to express mucins. This proposal seeks to clone and to characterize these genes, to define the level of regulation by vitamin A (i.e., transcription or translation), to elucidate the expression of these genes in airway epithelium, and finally to define the mechanism by which these genes induce mucous cell differentiation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37HL035635-11
Application #
2873443
Study Section
Special Emphasis Panel (ZRG2-RAP (01))
Project Start
1987-04-01
Project End
2000-05-31
Budget Start
1998-06-01
Budget End
1999-05-31
Support Year
11
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California Davis
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Kao, Cheng-Yuan; Kim, Christy; Huang, Fei et al. (2008) Requirements for two proximal NF-kappaB binding sites and IkappaB-zeta in IL-17A-induced human beta-defensin 2 expression by conducting airway epithelium. J Biol Chem 283:15309-18
Chen, Yin; Hamati, Edward; Lee, Pak-Kei et al. (2006) Rhinovirus induces airway epithelial gene expression through double-stranded RNA and IFN-dependent pathways. Am J Respir Cell Mol Biol 34:192-203
Thai, Philip; Chen, Yin; Dolganov, Gregory et al. (2005) Differential regulation of MUC5AC/Muc5ac and hCLCA-1/mGob-5 expression in airway epithelium. Am J Respir Cell Mol Biol 33:523-30
Kao, Cheng-Yuan; Huang, Fei; Chen, Yin et al. (2005) Up-regulation of CC chemokine ligand 20 expression in human airway epithelium by IL-17 through a JAK-independent but MEK/NF-kappaB-dependent signaling pathway. J Immunol 175:6676-85
Wachi, Shinichiro; Yoneda, Ken; Wu, Reen (2005) Interactome-transcriptome analysis reveals the high centrality of genes differentially expressed in lung cancer tissues. Bioinformatics 21:4205-8
Kao, Cheng-Yuan; Chen, Yin; Thai, Philip et al. (2004) IL-17 markedly up-regulates beta-defensin-2 expression in human airway epithelium via JAK and NF-kappaB signaling pathways. J Immunol 173:3482-91
Harper, Richart; Xu, Changhong; Di, Peter et al. (2004) Identification of a novel MAGE D2 antisense RNA transcript in human tissues. Biochem Biophys Res Commun 324:199-204
Oslund, Karen L; Miller, Lisa A; Usachenko, Jodie L et al. (2004) Oxidant-injured airway epithelial cells upregulate thioredoxin but do not produce interleukin-8. Am J Respir Cell Mol Biol 30:597-604
Chen, Yin; Zhao, Yu Hua; Kalaslavadi, Tejas Baba et al. (2004) Genome-wide search and identification of a novel gel-forming mucin MUC19/Muc19 in glandular tissues. Am J Respir Cell Mol Biol 30:155-65
Di, Yuan-Pu; Harper, Richart; Zhao, Yuhua et al. (2003) Molecular cloning and characterization of spurt, a human novel gene that is retinoic acid-inducible and encodes a secretory protein specific in upper respiratory tracts. J Biol Chem 278:1165-73

Showing the most recent 10 out of 52 publications