Abstract

The basic approach is to establish molecular-structural and structural-functional correlations, under the premise that structure can be best understood by observing its changes following specific molecular perturbations, and that function cannot be fully modeled without knowledge of the underlying structure. Past and planned projects in which changes in structural parameters due to perturbation of protein expression relate to the functional effects of the same perturbation offer examples of such correlations. A second approach, which was initiated with the current funding is to relate phylogeny with structure and protein expression in the critical transition from low chordates to vertebrates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Method to Extend Research in Time (MERIT) Award (R37)
Project #
5R37HL048093-16
Application #
7655264
Study Section
Special Emphasis Panel (NSS)
Program Officer
Przywara, Dennis
Project Start
1993-07-01
Project End
2013-06-30
Budget Start
2009-07-01
Budget End
2010-06-30
Support Year
16
Fiscal Year
2009
Total Cost
$352,425
Indirect Cost

  Institution

Name
University of Pennsylvania
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104

  Publications

Lavorato, Manuela; Huang, Tai-Qin; Iyer, Venkat Ramesh et al. (2015) Dyad content is reduced in cardiac myocytes of mice with impaired calmodulin regulation of RyR2. J Muscle Res Cell Motil 36:205-14
Franzini-Armstrong, Clara (2014) Memories of Annemarie Weber. Anat Rec (Hoboken) 297:1543-7
Park, Chang Sik; Chen, Shan; Lee, Hoyong et al. (2013) Targeted ablation of the histidine-rich Ca(2+)-binding protein (HRC) gene is associated with abnormal SR Ca(2+)-cycling and severe pathology under pressure-overload stress. Basic Res Cardiol 108:344
Dayal, Anamika; Schredelseker, Johann; Franzini-Armstrong, Clara et al. (2010) Skeletal muscle excitation-contraction coupling is independent of a conserved heptad repeat motif in the C-terminus of the DHPRbeta(1a) subunit. Cell Calcium 47:500-6
Schredelseker, Johann; Dayal, Anamika; Schwerte, Thorsten et al. (2009) Proper restoration of excitation-contraction coupling in the dihydropyridine receptor beta1-null zebrafish relaxed is an exclusive function of the beta1a subunit. J Biol Chem 284:1242-51
Di Maio, Alessandro; Block, Barbara A (2008) Ultrastructure of the sarcoplasmic reticulum in cardiac myocytes from Pacific bluefin tuna. Cell Tissue Res 334:121-34
Snopko, Rose M; Ramos-Franco, Josefina; Di Maio, Alessandro et al. (2008) Ca2+ sparks and cellular distribution of ryanodine receptors in developing cardiomyocytes from rat. J Mol Cell Cardiol 44:1032-44
Di Maio, Alessandro; Karko, Kimberly; Snopko, Rose M et al. (2007) T-tubule formation in cardiacmyocytes: two possible mechanisms? J Muscle Res Cell Motil 28:231-41
Di Maio, Alessandro; Ter Keurs, H E; Franzini-Armstrong, Clara (2007) T-tubule profiles in Purkinje fibres of mammalian myocardium. J Muscle Res Cell Motil 28:115-21
Chopra, Nagesh; Kannankeril, Prince J; Yang, Tao et al. (2007) Modest reductions of cardiac calsequestrin increase sarcoplasmic reticulum Ca2+ leak independent of luminal Ca2+ and trigger ventricular arrhythmias in mice. Circ Res 101:617-26

Showing the most recent 10 out of 14 publications