Our aim is to develop an inexpensive and portable nanotechnology-based immunosensor and instrument platform for rapid detection of presence or exposure to bioterror agents in air, water, contaminated surfaces, forensic samples, food and body fluids through identification of protein toxins and microorganisms. The proposed approach employs single or multiple conically shaped gold nanotubes embedded within a mechanical and chemically robust polymeric membrane. The sensing measurement entails passing an ionic electrolyte current through the nanotube. Analyte in the electrolyte (e.g., a protein or virus) binds to a biochemical molecular-recognition agent such as an antibody immobilized at the small-diameter opening (or mouth) of the conical nanotube, which blocks the ion current. The approach is technically capable of registering single molecule/particle events and as such can lead to extremely low levels of detection with high specificity. The method is highly adaptable to microfluidic platforms and can be adapted to microliter sample volumes. The Phase I program will extend feasibility studies already completed at the University of Florida to developing operational parameters and assessing instrument performance for detecting model toxins in drinking water. Such instruments are envisioned as real-time monitors in buildings, subways, etc ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Small Business Technology Transfer (STTR) Grants - Phase I (R41)
Project #
5R41AI068181-02
Application #
7191714
Study Section
Special Emphasis Panel (ZRG1-BST-G (91))
Program Officer
Hall, Robert H
Project Start
2006-03-01
Project End
2009-02-28
Budget Start
2007-03-01
Budget End
2009-02-28
Support Year
2
Fiscal Year
2007
Total Cost
$231,678
Indirect Cost
Name
Eic Laboratories, Inc.
Department
Type
DUNS #
076603836
City
Norwood
State
MA
Country
United States
Zip Code
02062