Head and Neck Squamous Cell Carcinoma (HNSCC) is a disease with significant morbidity and mortality, and without reliable indicators for prognosis, local recurrence, or presence of premalignant disease. This project is focused on validation of epigenetic, promoter methylation-based salivary rinse biomarkers that have been previously developed and published using a cohort of head and neck squamous cell carcinoma (HNSCC) patients and non-affected control subjects. Prior data shows these markers to be specific for head and neck cancer detection, and preliminary data show that detection of these markers in pretreatment salivary rinses in HNSCC patients is an independent prognostic indicator for local recurrence and poor survival. We will 1) define these markers in a separate validation cohort of salivary rinses from HNSCC patients to confirm association with local recurrence and poor overall survival. We will also 2) validate and test these markers in a clinical oral cancer detection cohort of malignant and premalignant lesions, respectively, previously collected under a U54 mechanism at the NYU College of Dentistry (U54DE014257, Dr. David Sirois).

Public Health Relevance

This project is focused on validation of epigenetic, promoter methylation-based salivary rinse biomarkers that have been previously developed and published using a cohort of head and neck squamous cell carcinoma (HNSCC) patients and non-affected control subjects.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
High Impact Research and Research Infrastructure Programs (RC2)
Project #
5RC2DE020789-02
Application #
7937951
Study Section
Special Emphasis Panel (ZDE1-MH (38))
Program Officer
Harris, Emily L
Project Start
2009-09-25
Project End
2012-08-31
Budget Start
2010-09-01
Budget End
2012-08-31
Support Year
2
Fiscal Year
2010
Total Cost
$265,486
Indirect Cost
Name
Johns Hopkins University
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
Gaykalova, Daria A; Manola, Judith B; Ozawa, Hiroyuki et al. (2015) NF-?B and stat3 transcription factor signatures differentiate HPV-positive and HPV-negative head and neck squamous cell carcinoma. Int J Cancer 137:1879-89
Gaykalova, Daria A; Mambo, Elizabeth; Choudhary, Ashish et al. (2014) Novel insight into mutational landscape of head and neck squamous cell carcinoma. PLoS One 9:e93102
Sun, Wenyue; Califano, Joseph A (2014) Sequencing the head and neck cancer genome: implications for therapy. Ann N Y Acad Sci 1333:33-42
Sun, Wenyue; Gaykalova, Daria A; Ochs, Michael F et al. (2014) Activation of the NOTCH pathway in head and neck cancer. Cancer Res 74:1091-104
Schussel, Juliana; Zhou, Xian Chong; Zhang, Zhe et al. (2013) EDNRB and DCC salivary rinse hypermethylation has a similar performance as expert clinical examination in discrimination of oral cancer/dysplasia versus benign lesions. Clin Cancer Res 19:3268-75
Sun, Wenyue; Zaboli, David; Wang, Hao et al. (2012) Detection of TIMP3 promoter hypermethylation in salivary rinse as an independent predictor of local recurrence-free survival in head and neck cancer. Clin Cancer Res 18:1082-91
Sun, Wenyue; Zaboli, David; Liu, Yan et al. (2012) Comparison of promoter hypermethylation pattern in salivary rinses collected with and without an exfoliating brush from patients with HNSCC. PLoS One 7:e33642