This proposal requests support for the purchase of a Betascope 603 Blot Analyzer-Image Analysis System. The instrument provides real time data processing and image construction of radioactive samples deposited on gels or blots routinely utilized in molecular biological research. As a replacement for autoradiography, the Betascope provides finely detailed images 20-25 times faster than conventional x-ray film. For example, blots or gels normally requiring overnight exposure are typically imaged with the Betascope within 30-45 min. Most importantly, the instrument has the ability to accurately quantitate levels of 32P over a linear response range of 5 orders of magnitude per 10 square mm thus permitting precise quantitation of individual bands, dots etc. By comparison, densitometer scans of autoradiographs have linear ranges of roughly 2 orders of magnitude. In addition, the system provides for superior data management capabilities. All of the numerical data from an analysis are stored as digitized information suitable for spreadsheet and graph analysis utilizing computer software also provided with the instrument. The Betascope will be a multiuser instrument utilized primarily by a group of 10 investigators within the Department of Cellular and Structural Biology. This group of major users are all supported by NIH grants; their total support for 1989 is more than $1.4 million in direct costs. In addition, the instrument will be available on a priority basis to other investigators within the institution who have NIH grant support or who are collecting preliminary data in order to apply for grant support.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biomedical Research Support Grants (S07)
Project #
2S07RR005807-13
Application #
3516614
Study Section
Special Emphasis Panel (NSS)
Project Start
1979-04-01
Project End
1993-09-29
Budget Start
1991-04-01
Budget End
1993-09-29
Support Year
13
Fiscal Year
1991
Total Cost
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
Schools of Optometry/Ophthalmol
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Marsh-Tootle, W L; Corliss, D A; Alvarez, S L et al. (1994) A statistical analysis of Modified Clinical Technique vision screening of preschoolers by optometry students. Optom Vis Sci 71:593-603
Wesson, M D; Bartlett, J D; Swiatocha, J et al. (1993) Mydriatic efficacy of a cycloplegic spray in the pediatric population. J Am Optom Assoc 64:637-40
Bartlett, J D; Wesson, M D; Swiatocha, J et al. (1993) Efficacy of a pediatric cycloplegic administered as a spray. J Am Optom Assoc 64:617-21
Bartlett, J D; Woolley, T W; Adams, C M (1993) Identification of high intraocular pressure responders to topical ophthalmic corticosteroids. J Ocul Pharmacol 9:35-45
Wiggins, N P; Daum, K M; Snyder, C A (1992) Effects of residual astigmatism in contact lens wear on visual discomfort in VDT use. J Am Optom Assoc 63:177-81
Semes, L (1991) UV-A absorbing characteristics of commercial sunglasses intended for recreational and general use. J Am Optom Assoc 62:754-8
Kuyk, T K; Wesson, M D (1991) Aging-related foveal flicker sensitivity losses in normal observers. Optom Vis Sci 68:786-9
Wiggins, N P; Daum, K M (1991) Visual discomfort and astigmatic refractive errors in VDT use. J Am Optom Assoc 62:680-4
Nowakowski, R W; Thompson, J N; Edge, D S (1990) A screening method for mucopolysaccharidoses with increased urinary excretion of sulfated N-acetylhexosamines. Biochem Int 22:419-26
Fuhr, P S; Hershner, T A; Daum, K M (1990) Ganzfeld blankout occurs in bowl perimetry and is eliminated by translucent occlusion. Arch Ophthalmol 108:983-8

Showing the most recent 10 out of 20 publications