This application requests the purchase of a Matrix-Assisted Laser Desorption Ionization (MALDI)-TOF-TOF (Time-of-Flight) mass spectrometry instrument for multi-investigator use in the Mass Spectrometry Laboratory (MSL) at the University of Illinois at Urbana- Champaign. The MSL serves a large number of departments at the University of Illinois and also receives many samples from outside. The requested instrument has greatly improved mass accuracy compared to our current MALDI instrument, and can deliver routine MS-MS data unlike our current instrument. Access to the requested state-of-the- art technology would enable many critical experiments in NIH-funded laboratories at the University of Illinois that cannot be performed at present. In particular, it will greatly aid the investigation of the biosynthesis of peptide antibiotics, the structural elucidation of cyclic antimicrobial peptides discovered from genome databases, the development of dendrimers as drug delivery vehicles, the development of important new sensors for volatile organic compounds, and the understanding of nucleic acid catalysis and molecular recognition. In addition, the requested instrument will be used to better understand metalloenzymes that are key to human health as well as to elucidate glycan biosynthetic pathways.
This application requests funds to purchase a state-of-the-art instrument that can accurately determine the weight of large biomolecules. In addition, the instrument can fragment the molecules and analyze the fragments, thereby providing further information that is key for biomedical scientists. The requested shared instrument will provide data for more than 100 scientists at the University of Illinois.
| Hegemann, Julian D; Schwalen, Christopher J; Mitchell, Douglas A et al. (2018) Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin. Chem Commun (Camb) 54:9007-9010 |
| Hegemann, Julian D; van der Donk, Wilfred A (2018) Investigation of Substrate Recognition and Biosynthesis in Class IV Lanthipeptide Systems. J Am Chem Soc 140:5743-5754 |
| Repka, Lindsay M; Hetrick, Kenton J; Chee, See Hyun et al. (2018) Characterization of Leader Peptide Binding During Catalysis by the Nisin Dehydratase NisB. J Am Chem Soc 140:4200-4203 |
| Bobeica, Silvia C; van der Donk, Wilfred A (2018) The Enzymology of Prochlorosin Biosynthesis. Methods Enzymol 604:165-203 |
| Hetrick, Kenton J; Walker, Mark C; van der Donk, Wilfred A (2018) Development and Application of Yeast and Phage Display of Diverse Lanthipeptides. ACS Cent Sci 4:458-467 |
| Kakkar, Nidhi; Perez, Jessica G; Liu, Wenshe R et al. (2018) Incorporation of Nonproteinogenic Amino Acids in Class I and II Lantibiotics. ACS Chem Biol 13:951-957 |
| Chekan, Jonathan R; Estrada, Paola; Covello, Patrick S et al. (2017) Characterization of the macrocyclase involved in the biosynthesis of RiPP cyclic peptides in plants. Proc Natl Acad Sci U S A 114:6551-6556 |
| Ortega, Manuel A; Cogan, Dillon P; Mukherjee, Subha et al. (2017) Two Flavoenzymes Catalyze the Post-Translational Generation of 5-Chlorotryptophan and 2-Aminovinyl-Cysteine during NAI-107 Biosynthesis. ACS Chem Biol 12:548-557 |
| Biswas, Subhanip; Garcia De Gonzalo, Chantal V; Repka, Lindsay M et al. (2017) Structure-Activity Relationships of the S-Linked Glycocin Sublancin. ACS Chem Biol 12:2965-2969 |
| Tietz, Jonathan I; Schwalen, Christopher J; Patel, Parth S et al. (2017) A new genome-mining tool redefines the lasso peptide biosynthetic landscape. Nat Chem Biol 13:470-478 |
Showing the most recent 10 out of 44 publications
