Role of Htlv Lor Region in Transcriptional Regulation

Sodroski, Joseph G.

Abstract

This proposal is aimed at understanding the role of the HTLV LOR production in transcriptional activation of specific target sequences, and how this process relates to the transforming ability of the virus. We wish to characterize the HTLV LOR product for subcellular location and DNA binding properties and to express it in prokaryotic and eukaryotic vector systems. We are specifically interested in expressing the LOR product in the absence of other HTLV related proteins to clearly define its role in trans-activation of the HTLV LTR and of particular cellular genes, as well as in the ability of HTLV to immortalize primary lymphocytes.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Research Project--Cooperative Agreements (U01)
• Project #: 1U01CA040658-01
• Application #: 3548613
• Study Section: (SRC)
• Project Start: 1985-07-01
• Project End: 1988-06-30
• Budget Start: 1985-07-01
• Budget End: 1986-06-30
• Support Year: 1
• Fiscal Year: 1985

Institution

• Name: Dana-Farber Cancer Institute
• DUNS #: 149617367
• City: Boston
• State: MA
• Country: United States

Publications

Poteat, H T; Chen, F Y; Kadison, P et al. (1990) Protein kinase A-dependent binding of a nuclear factor to the 21-base-pair repeat of the human T-cell leukemia virus type I long terminal repeat. J Virol 64:1264-70

Dokhelar, M C; Pickford, H; Sodroski, J et al. (1989) HTLV-I p27rex regulates gag and env protein expression. J Acquir Immune Defic Syndr 2:431-40

Poteat, H T; Kadison, P; McGuire, K et al. (1989) Response of the human T-cell leukemia virus type 1 long terminal repeat to cyclic AMP. J Virol 63:1604-11

Dokhelar, M C; Pickford, H; Sodroski, J et al. (1989) The potential for homeostatic regulation of the X region proteins of the human T cell leukemia virus type I. J Acquir Immune Defic Syndr 2:588-94

Terwilliger, E; Burghoff, R; Sia, R et al. (1988) The art gene product of human immunodeficiency virus is required for replication. J Virol 62:655-8

Walker, B D; Kowalski, M; Goh, W C et al. (1987) Inhibition of human immunodeficiency virus syncytium formation and virus replication by castanospermine. Proc Natl Acad Sci U S A 84:8120-4

Goh, W C; Sodroski, J G; Rosen, C A et al. (1987) Expression of the art gene protein of human T-lymphotropic virus type III (HTLV-III/LAV) in bacteria. J Virol 61:633-7

Kowalski, M; Potz, J; Basiripour, L et al. (1987) Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1. Science 237:1351-5

Rosen, C A; Sodroski, J G; Willems, L et al. (1986) The 3' region of bovine leukemia virus genome encodes a trans-activator protein. EMBO J 5:2585-9

Terwilliger, E; Sodroski, J G; Rosen, C A et al. (1986) Effects of mutations within the 3' orf open reading frame region of human T-cell lymphotropic virus type III (HTLV-III/LAV) on replication and cytopathogenicity. J Virol 60:754-60