Abstract

This proposal is aimed at understanding the role of the HTLV LOR production in transcriptional activation of specific target sequences, and how this process relates to the transforming ability of the virus. We wish to characterize the HTLV LOR product for subcellular location and DNA binding properties and to express it in prokaryotic and eukaryotic vector systems. We are specifically interested in expressing the LOR product in the absence of other HTLV related proteins to clearly define its role in trans-activation of the HTLV LTR and of particular cellular genes, as well as in the ability of HTLV to immortalize primary lymphocytes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project--Cooperative Agreements (U01)
Project #
5U01CA040658-03
Application #
3548615
Study Section
(SRC)
Project Start
1985-07-01
Project End
1988-06-30
Budget Start
1987-07-01
Budget End
1988-06-30
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Dana-Farber Cancer Institute
Department
Type
DUNS #
149617367
City
Boston
State
MA
Country
United States
Zip Code
02115

  Publications

Poteat, H T; Chen, F Y; Kadison, P et al. (1990) Protein kinase A-dependent binding of a nuclear factor to the 21-base-pair repeat of the human T-cell leukemia virus type I long terminal repeat. J Virol 64:1264-70
Dokhelar, M C; Pickford, H; Sodroski, J et al. (1989) HTLV-I p27rex regulates gag and env protein expression. J Acquir Immune Defic Syndr 2:431-40
Poteat, H T; Kadison, P; McGuire, K et al. (1989) Response of the human T-cell leukemia virus type 1 long terminal repeat to cyclic AMP. J Virol 63:1604-11
Dokhelar, M C; Pickford, H; Sodroski, J et al. (1989) The potential for homeostatic regulation of the X region proteins of the human T cell leukemia virus type I. J Acquir Immune Defic Syndr 2:588-94
Terwilliger, E; Burghoff, R; Sia, R et al. (1988) The art gene product of human immunodeficiency virus is required for replication. J Virol 62:655-8
Walker, B D; Kowalski, M; Goh, W C et al. (1987) Inhibition of human immunodeficiency virus syncytium formation and virus replication by castanospermine. Proc Natl Acad Sci U S A 84:8120-4
Goh, W C; Sodroski, J G; Rosen, C A et al. (1987) Expression of the art gene protein of human T-lymphotropic virus type III (HTLV-III/LAV) in bacteria. J Virol 61:633-7
Kowalski, M; Potz, J; Basiripour, L et al. (1987) Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1. Science 237:1351-5
Rosen, C A; Sodroski, J G; Willems, L et al. (1986) The 3' region of bovine leukemia virus genome encodes a trans-activator protein. EMBO J 5:2585-9
Terwilliger, E; Sodroski, J G; Rosen, C A et al. (1986) Effects of mutations within the 3' orf open reading frame region of human T-cell lymphotropic virus type III (HTLV-III/LAV) on replication and cytopathogenicity. J Virol 60:754-60

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