A growing body of evidence indicates that the enzyme DNA topoisomerase II is an important target for the anti-tumor effect of a diverse group of drugs including intercalating agents and epipodophyllotoxins. The important properties common to all these agents which allow them to interact with the enzyme are unknown. Based on the fact that the known active agents are structurally diverse, possess important clinical anti-tumor activity and share a common intracellular target, we believe it is highly likely that other compounds exist which, by virtue of their interaction with topoisomerase, will exhibit useful anti-tumor activity. Thus, the goals of our program are (1) to elucidate the nature of the physical interaction of topoisomerase II with specific, active anti-tumor agents; (2) to explore the potential of DNA topoisomerase I as a therapeutic target; (3) to design new agents directed at topoisomerase I and II; (4) to develop sensitive, efficient assays for screening new compounds for activity against topoisomerases I and II and (5) to characterize topoisomerases in vivo in ways which will elucidate the biological bases for therapeutic success or failure.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project--Cooperative Agreements (U01)
Project #
1U01CA040884-01
Application #
3548698
Study Section
(SRC)
Project Start
1985-07-01
Project End
1990-06-30
Budget Start
1985-07-01
Budget End
1986-06-30
Support Year
1
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Florida
Department
Type
Schools of Medicine
DUNS #
073130411
City
Gainesville
State
FL
Country
United States
Zip Code
32611
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