NIA Supplement to U01DA047713: Defining novel PTPRD roles in Alzheimer's disease tau pathophysiology George R Uhl MD PhD PI Project Summary/Abstract Pathological tau hyperphosphorylation and neurofibrillary pathology are major Alzheimer's disease (AD) pathophysiological elements that correlate with clinical dementia. Activities of several kinases that hyperphosphorylate tau, including cyclin dependent CDK5, glycogen synthase GSK3? and GSK3? are enhanced by phosphorylation of their own tyrosines (pY15, pY279 and pY216). Tyrosine kinases that phosphorylate and activate these three tau-phosphorylating kinases are known. However, the tyrosine phosphatase(s) that dephosphorylate and thus reduce activities of brain CDK5, GSK3? and GSK3? have not been reported. Several lines of evidence now support PTPRD, a receptor type protein tyrosine phosphatase, as a major contributor to dephosphorylating tau-phosphorylating enzymes including CDK5, GSK3? and GSK3?. We will test the hypothesis that PTPRD contributes to regulation of CDK5, GSK3? and GSK3? activities in ways that make PTPRD a novel, multifactorial, druggable contributor to tau pathophysiology in AD. We are fortunate that work developing a PTPRD phosphatase inhibitor to reduce reward from addictive substances is funded by a NIDA parent grant U01DA047713 that provides an exceptionally solid platform for the current supplement proposal. In vitro, we will test activities of CDK5, GSK3? and GSK3? wildtype, mutant and control/comparison phosphopeptides at wildtype/mutant human D1 and D1 + D2 phosphatase domains from PTPRD and other tyrosine phosphatases. We will seek potent interactions between wildtype PTPRD phosphatase and pY15 CDK5, pY279 GSK3? and/or pY216 GSK3? phosphopeptides vs lower potency interactions with dephosphorylated/mutant/control peptides, PTPRD mutants and other tyrosine phosphatases. In vivo, we will characterize effects of genetically or pharmacologically altered PTPRD activity on brain CDK5, GSK3? and GSK3? tyrosine phosphorylation in mice. In pilot projects, we will a) cross 3xTg-AD mice with PTPRD knockouts to seek suitability of these ?4xTg-AD? mice for studies of effects on tau pathology in aging and b) synthesize and model NF504, NF506 and other potential positive allosteric modulators of PTPRD's phosphatase for future tests of our innovative hypotheses. This work will aid new understanding of Alzheimer's disease neurofibrillary pathology and help to define PTPRD as a novel therapeutic target.
We will test the novel idea that PTPRD modulates the activities of brain enzymes that hyperphosphorylate tau, alters neurofibrillary pathology and changes vulnerability to Alzheimer's disease dementia. We will study in vitro effects of PTPRD on these enzymes and examine in vivo how modulating PTPRD influences these enzymes. These results and pilot projects will provide new insights and reagents as we (likely) validate PTPRD as a novel therapeutic target for Alzheimer's disease tau pathology.