The overall objectives of this T cell immortalization and cell immunology core, termed Core B, are to provide the following services and functions for all of the projects and investigators in the Center. These services will include: (1) a central resource for the establishment of immortalized T cell lines and clones from PBL, biopsy sites of inflammation (skin, synovium, kidney, lymph nodes, bone marrow) and inflammatory fluids (synovial, pleural, bronchoalveolar lavage and CSF) in patients with autoimmune disease. We will also establish bank of immortalized and well characterized functional libraries of T cells that may be of value to investigators and to other investigators in the autoimmune network. T cells will be immortalized by Herpes Virus Saimiri (HVS) HVS immortalized T cells can be grown with out the requirement for continual triggering with antigen, antigen presenting cells and IL-2. (2) central resource for the establishment of IL-2 dependent antigen specific T cell clones from PBL and tissues from patients with autoimmunity disease. (3) a cellular immunology service to evaluate both immortalized T cell clones, as well as IL-2 dependent T cell lines and peripheral blood T cells with respect to conventional functional and phenotype assays of antigen specificity (including augmentation of classical activation markers of T cells including CD40L, CD25, CD69 and MHC class II) as well as by chemokine and lymphokine release by ELISA and CTL function. (4) a central resource laboratory for the immortalization of B cells by EBV infection in all patients studied in our center. These cells will be used for MHC typing in Dr. Winchester's laboratory and as a potential source for antigen presenting cells in many of the T cell function studies planned in our center.

National Institute of Health (NIH)
National Institute of Allergy and Infectious Diseases (NIAID)
Research Program--Cooperative Agreements (U19)
Project #
Application #
Study Section
Special Emphasis Panel (ZAI1-EWS-I (S1))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Columbia University (N.Y.)
New York
United States
Zip Code
Winchester, Robert; Wiesendanger, Margrit; Zhang, Hui-Zhu et al. (2012) Immunologic characteristics of intrarenal T cells: trafficking of expanded CD8+ T cell ?-chain clonotypes in progressive lupus nephritis. Arthritis Rheum 64:1589-600
Ablamunits, Vitaly; Henegariu, Octavian; Preston-Hurlburt, Paula et al. (2011) NKG2A is a marker for acquisition of regulatory function by human CD8+ T cells activated with anti-CD3 antibody. Eur J Immunol 41:1832-42
Jiang, Hong; Canfield, Steve M; Gallagher, Mary P et al. (2010) HLA-E-restricted regulatory CD8(+) T cells are involved in development and control of human autoimmune type 1 diabetes. J Clin Invest 120:3641-50
Wu, Yilun; Zheng, Zongyu; Jiang, Yihua et al. (2009) The specificity of T cell regulation that enables self-nonself discrimination in the periphery. Proc Natl Acad Sci U S A 106:534-9
Jiang, Hong; Chess, Leonard (2009) How the immune system achieves self-nonself discrimination during adaptive immunity. Adv Immunol 102:95-133
Jiang, Hong; Chess, Leonard (2008) Qa-1/HLA-E-restricted regulatory CD8+ T cells and self-nonself discrimination: an essay on peripheral T-cell regulation. Hum Immunol 69:721-7
Chen, Weiling; Zhang, Linging; Liang, Bitao et al. (2007) Perceiving the avidity of T cell activation can be translated into peripheral T cell regulation. Proc Natl Acad Sci U S A 104:20472-7
Wu, Henry D; Maurer, Mathew S; Friedman, Richard A et al. (2007) The lymphocytic infiltration in calcific aortic stenosis predominantly consists of clonally expanded T cells. J Immunol 178:5329-39
Ben-Horin, Shomron; Green, Peter H R; Bank, Ilan et al. (2006) Characterizing the circulating, gliadin-specific CD4+ memory T cells in patients with celiac disease: linkage between memory function, gut homing and Th1 polarization. J Leukoc Biol 79:676-85
Tsai, E B; Sherry, N A; Palmer, J P et al. (2006) The rise and fall of insulin secretion in type 1 diabetes mellitus. Diabetologia 49:261-70

Showing the most recent 10 out of 32 publications