This core will provide services to University of Chicago Autoimmunity Center of Excellence (UCACE) investigators to characterize the role of B lymphocytes in autoimmune pathology. There are two state-of-the-art technologies that will allow the center investigators to scrutinize autoantibody-mediated pathology. 1. Analysis of B lymphocyte specificity using recombinant monoclonal (mAb) technology. 2. Immunoglobulin light chain variable gene microarrays Finally, Drs. Weigert and Wilson will assist and provide technical support for studies of the immunoglobulin repertoire using single-cell PCR, cloning and sequencing. This approach will augment the use of light chain microarrays and human mAb analysis. The unique methodologies for scrutinizing human B cell mediated pathology will provide powerful tools to the center to facilitate our understanding of lupus and myasthenia gravis.

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Research Program--Cooperative Agreements (U19)
Project #
5U19AI082724-03
Application #
8301738
Study Section
Special Emphasis Panel (ZAI1)
Project Start
Project End
Budget Start
2011-06-01
Budget End
2012-05-31
Support Year
3
Fiscal Year
2011
Total Cost
$210,344
Indirect Cost
Name
University of Chicago
Department
Type
DUNS #
005421136
City
Chicago
State
IL
Country
United States
Zip Code
60637
Kinloch, Andrew J; Kaiser, Ylva; Wolfgeher, Don et al. (2018) In Situ Humoral Immunity to Vimentin in HLA-DRB1*03+ Patients With Pulmonary Sarcoidosis. Front Immunol 9:1516
Chen, Yao-Qing; Wohlbold, Teddy John; Zheng, Nai-Ying et al. (2018) Influenza Infection in Humans Induces Broadly Cross-Reactive and Protective Neuraminidase-Reactive Antibodies. Cell 173:417-429.e10
Henry, Carole; Palm, Anna-Karin E; Krammer, Florian et al. (2018) From Original Antigenic Sin to the Universal Influenza Virus Vaccine. Trends Immunol 39:70-79
Wilson, Patrick C; Cobey, Sarah (2018) Characterization of the immunologic repertoire: A quick start guide. Immunol Rev 284:5-8
Stamper, Christopher T; Wilson, Patrick C (2018) What Are the Primary Limitations in B-Cell Affinity Maturation, and How Much Affinity Maturation Can We Drive with Vaccination? Is Affinity Maturation a Self-Defeating Process for Eliciting Broad Protection? Cold Spring Harb Perspect Biol 10:
DiPiazza, Anthony; Nogales, Aitor; Poulton, Nicholas et al. (2017) Pandemic 2009 H1N1 Influenza Venus reporter virus reveals broad diversity of MHC class II-positive antigen-bearing cells following infection in vivo. Sci Rep 7:10857
Lau, Denise; Lan, Linda Yu-Ling; Andrews, Sarah F et al. (2017) Low CD21 expression defines a population of recent germinal center graduates primed for plasma cell differentiation. Sci Immunol 2:
Bunker, Jeffrey J; Erickson, Steven A; Flynn, Theodore M et al. (2017) Natural polyreactive IgA antibodies coat the intestinal microbiota. Science 358:
Rivas, Jacqueline R; Ireland, Sara J; Chkheidze, Rati et al. (2017) Peripheral VH4+ plasmablasts demonstrate autoreactive B cell expansion toward brain antigens in early multiple sclerosis patients. Acta Neuropathol 133:43-60
Zost, Seth J; Parkhouse, Kaela; Gumina, Megan E et al. (2017) Contemporary H3N2 influenza viruses have a glycosylation site that alters binding of antibodies elicited by egg-adapted vaccine strains. Proc Natl Acad Sci U S A 114:12578-12583

Showing the most recent 10 out of 51 publications