The purpose of this project is to map the locations at which specific genes are expressed in pre-implantation mouse embryos. Dr. Minoru Ko's group in LG has used expression profiling with microarrays to identify 500-1000 genes that are differentially expressed at various stages of early mouse embryo development. Initially, fluorescence in-situ hybridization (FISH) will be used to probe the locations within the embryo where these genes are expressed. Three dimensional restoration microscopy (the DeltaVision system from Applied Precision, Inc) will be employed to image these fluorescent probes. In conjunction with other fluorescent cellular markers for DNA, cytoskeletal components, and membranes, we plan to build a 3-dimensional model of the early mouse embryo in a standardized orientation, so that we can compare the location and level of gene expression from different imaging experiments. Eventually a database will be constructed to link the imaging experiments to microarray data and other mouse genomics data sources. We have recently demonstrated the applicability of DeltaVision as an imaging system for these experiments, and are currently evaluating fluorescent probe technology for use in FISH.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Intramural Research (Z01)
Project #
1Z01AG000670-01
Application #
6668442
Study Section
(LG)
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2002
Total Cost
Indirect Cost
Name
Aging
Department
Type
DUNS #
City
State
Country
United States
Zip Code
Yoshikawa, Toshiyuki; Piao, Yulan; Zhong, Jinhui et al. (2006) High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization. Gene Expr Patterns 6:213-24