During the last year, we have generated 60 ES cell lines, in each of which one of a total of 60 different TFs can be overexpressed in tetracycline-inducible manner. We have characterized these ES cell lines as follows: (i) subcellular localization of Flag-tagged transcription factors by immunohistochemistry; (ii) induction levels of the manipulated transcription factors by quantitative RT-PCR, (iii) DNA microarray-based expression profiling before and after induction of the transcription factors; (iv) western blotting, and (v) karyotyping. Together, these results indicate that we have generated truly reliable TF-manipulable ES cell lines. We are currently expanding the collection of ES cell lines.
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| Sharov, Alexei A; Masui, Shinji; Sharova, Lioudmila V et al. (2008) Identification of Pou5f1, Sox2, and Nanog downstream target genes with statistical confidence by applying a novel algorithm to time course microarray and genome-wide chromatin immunoprecipitation data. BMC Genomics 9:269 |
| Carter, Mark G; Stagg, Carole A; Falco, Geppino et al. (2008) An in situ hybridization-based screen for heterogeneously expressed genes in mouse ES cells. Gene Expr Patterns 8:181-98 |
