Recombinant Protein Expression Unit (RPEU)

Miller, Louis

Abstract

The Malaria Vaccine and Development Unit (MVDU) has as its goal the development of candidate vaccines to the point of testing in volunteers and in endemic people and the exploration of the next ideas for testing for vaccine development (the pipeline). The process development will proceed within the unit, and the clinical grade material will be outsourced for production. The unit will be able to produce recombinant proteins by fermentation in bacteria and yeast (Pichia and Saccharomyces) and do cell culture; purify proteins by methods that will be transferable for GMP production; and develop assays for release specifications and for evaluating the efficacy of proteins. It will also have a unit to do tests of recombinant proteins in Aotus monkeys and in field trials for efficacy.There are now three products that are approaching readiness for testing. Two are related to transmission blocking vaccines for P. falciparum and P. vivax and one is against the C-terminus of MSP1. The MSP1 vaccines are being made in different ways and will soon be tested in monkeys in preparation for field trials. - Malaria vaccine, blood stage vaccines, transmission-blocking vaccine - Human Subjects

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Allergy and Infectious Diseases (NIAID)
• Type: Intramural Research (Z01)
• Project #: 1Z01AI000727-05
• Application #: 6288945
• Study Section: Special Emphasis Panel (LPD)
• Support Year: 5
• Fiscal Year: 1999

Institution

• Name: National Institute of Allergy and Infectious Diseases
• Country: United States

Publications

Shimp Jr, Richard L; Martin, Laura B; Zhang, Yanling et al. (2006) Production and characterization of clinical grade Escherichia coli derived Plasmodium falciparum 42 kDa merozoite surface protein 1 (MSP1(42)) in the absence of an affinity tag. Protein Expr Purif 50:58-67

Singh, Sanjay; Miura, Kazutoyo; Zhou, Hong et al. (2006) Immunity to recombinant plasmodium falciparum merozoite surface protein 1 (MSP1): protection in Aotus nancymai monkeys strongly correlates with anti-MSP1 antibody titer and in vitro parasite-inhibitory activity. Infect Immun 74:4573-80

Collins, William E; Barnwell, John W; Sullivan, Joann S et al. (2006) Assessment of transmission-blocking activity of candidate Pvs25 vaccine using gametocytes from chimpanzees. Am J Trop Med Hyg 74:215-21

Tsai, Chiawei W; Duggan, Peter F; Shimp Jr, Richard L et al. (2006) Overproduction of Pichia pastoris or Plasmodium falciparum protein disulfide isomerase affects expression, folding and O-linked glycosylation of a malaria vaccine candidate expressed in P. pastoris. J Biotechnol 121:458-70

Makobongo, Morris O; Keegan, Brian; Long, Carole A et al. (2006) Immunization of Aotus monkeys with recombinant cysteine-rich interdomain region 1 alpha protects against severe disease during Plasmodium falciparum reinfection. J Infect Dis 193:731-40

Wille-Reece, Ulrike; Flynn, Barbara J; Lore, Karin et al. (2006) Toll-like receptor agonists influence the magnitude and quality of memory T cell responses after prime-boost immunization in nonhuman primates. J Exp Med 203:1249-58

Giersing, Birgitte K; Dubovsky, Filip; Saul, Allan et al. (2006) Potency assay design for adjuvanted recombinant proteins as malaria vaccines. Vaccine 24:4264-70

Trinh, Loc; Phue, Je-Nie; Jaluria, Pratik et al. (2006) Screen-less expanded bed column: new approach for the recovery and purification of a malaria transmission blocking vaccine candidate from Pichia pastoris. Biotechnol Lett 28:951-8

Saxena, Ajay K; Singh, Kavita; Su, Hua-Poo et al. (2006) The essential mosquito-stage P25 and P28 proteins from Plasmodium form tile-like triangular prisms. Nat Struct Mol Biol 13:90-1

Mullen, Gregory E D; Giersing, Birgitte K; Ajose-Popoola, Olubunmi et al. (2006) Enhancement of functional antibody responses to AMA1-C1/Alhydrogel, a Plasmodium falciparum malaria vaccine, with CpG oligodeoxynucleotide. Vaccine 24:2497-505