Project 2: Overhauser enhanced Magnetic Resonance Imaging. (OMRI) We have examined several tumor models in mice to evaluate the image enhancement profiles. Different tumor types were grown in mice and OMRI experiments were carried out using Oxo 63 as the contrast agent when tumors were at the size of 1 ml. Enhancement profiles were found to be significantly varied in the tumors examined. The melanoma cell line B16 exhibited the highest enhancement where as the SCC cell line (squamous cell carcinoma) exhibited the least enhancement. Other cell lines such as the RIF-1, HT-29 exhibited intermediate enhancement. Additional experiments with conventional contrast enhanced MRI using Gd3+-DTPA on these animals with different tumors showed time -intensity profiles in images which had different behavior in each tumor type tested. Histologic studies quantitating the microvessel density in tumors are proposed to be carried out to study the dependence of image enhancement vs tumor perfusion.

National Institute of Health (NIH)
Division of Basic Sciences - NCI (NCI)
Intramural Research (Z01)
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Basic Sciences
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Matsumoto, Shingo; Saito, Keita; Yasui, Hironobu et al. (2013) EPR oxygen imaging and hyperpolarized 13C MRI of pyruvate metabolism as noninvasive biomarkers of tumor treatment response to a glycolysis inhibitor 3-bromopyruvate. Magn Reson Med 69:1443-50
Matsumoto, Shingo; Batra, Sonny; Saito, Keita et al. (2011) Antiangiogenic agent sunitinib transiently increases tumor oxygenation and suppresses cycling hypoxia. Cancer Res 71:6350-9
Hyodo, Fuminori; Murugesan, Ramachandran; Matsumoto, Ken-ichiro et al. (2008) Monitoring redox-sensitive paramagnetic contrast agent by EPRI, OMRI and MRI. J Magn Reson 190:105-12
Matsumoto, Ken-ichiro; Subramanian, Sankaran; Murugesan, Ramachandran et al. (2007) Spatially resolved biologic information from in vivo EPRI, OMRI, and MRI. Antioxid Redox Signal 9:1125-41
Hama, Yukihiro; Bernardo, Marcelino; Regino, Celeste A S et al. (2007) MR lymphangiography using dendrimer-based contrast agents: a comparison at 1.5T and 3.0T. Magn Reson Med 57:431-6
Matsumoto, Ken-ichiro; Bernardo, Marcelino; Subramanian, Sankaran et al. (2006) MR assessment of changes of tumor in response to hyperbaric oxygen treatment. Magn Reson Med 56:240-6
Matsumoto, Shingo; Utsumi, Hideo; Aravalluvan, Thirumaran et al. (2005) Influence of proton T1 on oxymetry using Overhauser enhanced magnetic resonance imaging. Magn Reson Med 54:213-7
Subramanian, Sankaran; Matsumoto, Ken-Ichiro; Mitchell, James B et al. (2004) Radio frequency continuous-wave and time-domain EPR imaging and Overhauser-enhanced magnetic resonance imaging of small animals: instrumental developments and comparison of relative merits for functional imaging. NMR Biomed 17:263-94